L5158
白血病抑制因子 来源于小鼠
≥95% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture
别名:
LIF
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关于此项目
Recombinant:
expressed in E. coli
Assay:
≥95% (SDS-PAGE)
Form:
buffered aqueous solution
产品名称
白血病抑制因子 来源于小鼠, LIF, recombinant, expressed in E. coli, 10 μg/ml, buffered aqueous solution, suitable for cell culture
recombinant
expressed in E. coli
Quality Level
assay
≥95% (SDS-PAGE)
form
buffered aqueous solution
quality
endotoxin tested
technique(s)
cell culture | mammalian: suitable
storage temp.
2-8°C
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Application
来自小鼠的白血病抑制因子已用于：
- 作为共培养加富培养基组分
- 作为培养心肌细胞的杜氏改良Eagle培养基(DMEM)/F12培养基的补充剂
- 作为维持胚胎干细胞生长的维持培养基组分
- 处理卵巢培养物的卵巢
Biochem/physiol Actions
白血病抑制因子（LIF）是一种多效性糖蛋白，它能够抑制小鼠骨髓白血病细胞系M1的增殖，同时诱导其分化成巨噬细胞。人和小鼠LIF具有78％的序列同源性。人LIF可以激活小鼠细胞，但小鼠LIF不能激活人体细胞。
白血病抑制因子（LIF）是一种多效性糖蛋白，最初被认为能够抑制小鼠骨髓白血病细胞系M1的增殖，同时诱导其分化成巨噬细胞。后来鉴定出LIF的其他活性，已知LIF具有多种别名，包括DIF、D因子、DIA、DRF、CNDF、HILDA、HSF-III和MLPLI。人LIF通过一个受体发挥作用，该受体包含一条190 kDa LIF-结合α-链（130 kDa，小鼠）和一条130 kDa信号传导β-链（gp130），它是CNTF、OSM、L-6和IL-11的共同受体。因此，它是细胞因子受体超家族的gp130家族的成员。已在多种细胞上鉴定了LIF受体，包括单核细胞、肝脏细胞、胎盘细胞和胚胎干细胞。天然LIF是高度糖基化的，表观分子量为32kDa至62kDa，具体取决于来源，但是无糖基化似乎不影响其生物活性。LIF由单个基因编码，它在人或小鼠中分泌为含有180个氨基酸的单链糖蛋白并具有保守二硫键。人和小鼠LIF具有78％的序列同源性。人LIF可以激活小鼠细胞，但小鼠LIF不能激活人体细胞。
Physical form
溶于含有0.02％TWEEN® 20的磷酸盐缓冲盐水
Analysis Note
利用M1小鼠细胞培养物检测小鼠LIF的增殖活性。
Legal Information
TWEEN is a registered trademark of Croda International PLC
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
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A Rodríguez et al.
Theriogenology, 67(5), 1092-1095 (2007-01-11)
Leukemia inhibitory factor (LIF) is a cytokine that shows conflicting effects on in vitro produced (IVP) bovine embryos. Bovine LIF (bLIF) has been cloned and used in culture, but there is no commercially available bLIF. Thus, researchers use human LIF
Suppressor of cytokine signaling 4 (SOCS4): moderator of ovarian primordial follicle activation
Sutherland JM, et al.
Journal of Cellular Physiology, 227(3), 1188-1198 (2012)
LIF-immobilized nonwoven polyester fabrics for cultivation of murine embryonic stem cells
Cetinkaya G, et al.
Journal of Biomedical Materials Research Part A, 81(4), 911-919 (2007)
Renée F de Pooter et al.
Methods in molecular biology (Clifton, N.J.), 330, 113-121 (2006-07-19)
Mature hematopoietic cells, like all other terminally differentiated lineages, arise during ontogeny via a series of increasingly restricted intermediates. Hematopoietic progenitors have their origin in the mesoderm, which gives rise to hemangioblasts that can differentiate into endothelial or endocardial precursors
商品
Leukemia Inhibitory Factor (LIF) is a stem cell growth factor used for the in vitro culture of pluripotent mouse embryonic stem cells (ES cells).
相关内容
Instructions
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| L5158-10UG | 04061833256060 |
| L5158-5UG | 04061833957943 |
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