产品名称
来自西非单叶豆（加纳籽）的凝集素, Isolectin B4 (BSI-B4), peroxidase conjugate, lyophilized powder
biological source
Bandieraea simplicifolia
conjugate
peroxidase conjugate
assay
≥90% protein basis (Warburg-Christian)
form
lyophilized powder
potency
<200 μg per mL agglutination activity (using human blood group B erythrocytes)
peroxidase activity
40-160 units/mg protein
composition
Protein, ~95% modified Warburg-Christian
technique(s)
blood typing: suitable
fractionation: suitable
storage temp.
−20°C
Quality Level
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Analysis Note
凝集活性以 μg/mL表示，并且使用磷酸盐缓冲盐水（pH6.8）对1mg/mL溶液进行连续稀释液来测定，对于每种凝集素，所含有钙、镁和锰的浓度不同。该活性是在25℃孵育1小时后，对2％合适的红细胞悬浮液进行凝集所需的最低浓度。
Application
通用蛋白质印迹方案：
- 糖蛋白样品规格：500ng
- 凝集素浓度： 0.1ug/ml
- 每条泳道上样500 ng糖蛋白样品
- 进行4-20% Bis-Tris SDS page凝胶
- 转膜凝胶至PVDF膜上
- 用于RIPA缓冲液（R0278 Sigma）在室温对膜进行封闭1小时
- 在室温用RIPA缓冲液以0.1 ug/ml孵育HRP凝集素2小时
- 用25ml RIPA缓冲液洗膜 5 x 5分钟
- 用化学发光底物（CPS1-120）进行检测
Biochem/physiol Actions
BS-I对末端α-D-半乳糖残基具有主要亲和力，对末端N-乙酰基-α-D-半乳糖胺残基具有二级亲和力。
Other Notes
BS-I是一种四聚体凝集素，由两种类型的亚基A和B组成。存在由不同亚基组成的五种BS-I同工凝集素：BSI-B4、BSI-AB3、BSI-A2B2、BSI-A3B、以及BSI-A4。BSI-B4 是血型B特异的，其对末端α-D-半乳糖残基具有独特的亲和力，而BSI-A4 具有血型A特异性，其对末端N-乙酰基-α-D-半乳糖胺残基具有主要亲和力。
在20℃、pH值为6.0条件下，1个单位的连苯三酚在20 s内会形成1 mg红棓酚。
Physical form
含柠檬酸钠缓冲盐和氯化钙
Preparation Note
使用O′Sullivan等人的方法的改进，由VI型过氧化物酶制备。偶联后通过亲和层析重新纯化。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
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