form
liquid
usage
sufficient for 100 reactions, sufficient for 2000 reactions, sufficient for 500 reactions
feature
dNTPs included, hotstart
Quality Level
technique(s)
RT-qPCR: suitable, qPCR: suitable
color
colorless
input
purified DNA
application(s)
agriculture
compatibility
Bio-Rad MyiQ, Bio-Rad iCycler iQ, Bio-Rad iQ 5, for use with ABI 5700, for use with ABI 7000, for use with ABI 7300, for use with ABI 7500 Fast, for use with ABI 7500, for use with ABI 7700, for use with ABI 7900 Fast, for use with ABI 7900 HT, for use with ABI 7900, for use with ABI StepOne, for use with ABI StepOnePlus, for use with ABI ViiA 7, for use with Bio-Rad CFX384, for use with Bio-Rad CFX96, for use with Bio-Rad MJ Chromo4, for use with Bio-Rad MJ Opticon 2, for use with Bio-Rad MJ Opticon Cepheid SmartCycler, for use with Bio-Rad MJ Opticon, for use with Bio-Rad MiniOpticon, for use with Eppendorf® Mastercycler ep realplex2 s, for use with Eppendorf® Mastercycler ep realplex, for use with Illumina Eco qPCR, for use with Qiagen Corbett Rotor-Gene 3000, for use with Qiagen Corbett Rotor-Gene 6000, for use with Qiagen Corbett Rotor-Gene Q, for use with Roche LightCycler 480, for use with Strategene Mx3000P, for use with Strategene Mx3005P, for use with Strategene Mx4000
detection method
SYBR® Green
shipped in
wet ice
storage temp.
−20°C
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General description
- Kit is designed to perform SYBR Green-based qPCR assays on commonly available real-time instrument platforms
- ReadyMix requires the addition of reference dye (provided in kit) when being used on real-time instruments that require ROX passive reference dye for normalization of qPCR assays
- Kit is not compatible with qPCR instruments that utilize glass capillary tubes
Application
Features and Benefits
- Assay results in as little as 25 minutes
- Unsurpassed accuracy, precision, and sensitivity
- Virtually eliminates the need to optimize assay parameters
- Ideal for performing high-throughput, quantitative PCR applications
- The master mix allows consistency from one reaction to the next
- Reduced preparation time and reduced risk of contamination from multiple pipetting steps
- The hot start mechanism using the JumpStart Taq antibody prevents amplification of non-specific products, resulting in increased efficiency and higher target yield
- LuminoCt® ReadyMixes require the use of small amplicons (<200bp) for optimal results
- This master mix is compatible with commercial primer sets, including TaqMan® assays
Packaging
100RXN is packaged as 1 X 2.5 mL
500RXN is packaged as 1 X 12.5 mL
2000RXN is packaged as 1 X 50 mL
Other Notes
- At a minimum, use software to design primers for assays and ensure that qPCR amplicons are <200 bp
- Sigma recommends designing primers and probes with Beacon Designer software
- To order primers and probes from Sigma, visit: www.sigma.com/oligos
Legal Information
仅试剂盒组分
- 2 X
存储类别
10 - Combustible liquids
wgk
WGK 1
法规信息
商品
PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.
The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension
聚合酶链反应是分子生物学应用最普遍的技术之一。PCR过程主要由三步组成：变性、退火和延伸
实验方案
Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions
我们的 SYBR Green qPCR 协议是专为检测基因表达和 RT-PCR 反应的精确定量而设计的方法。
相关内容
RT-qPCR detects specific targets with applications in gene expression and pathogen detection.
SYBR® Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. Explore our LuminoCt® and KiCqStart® products for Fast qPCR or JumpStart™ reagents for conventional qPCR
Instructions
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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