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Merck
CN

L6632

脂肪氧化酶 来源于大豆

Type V, ammonium sulfate suspension, 500,000-1,000,000 units/mg protein

别名:

亚油酸:氧氧化还原酶, 脂氧合酶

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关于此项目

化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-853-1
MDL number:
Specific activity:
500,000-1,000,000 units/mg protein
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产品名称

脂肪氧化酶 来源于大豆, Type V, ammonium sulfate suspension, 500,000-1,000,000 units/mg protein

type

Type V

form

ammonium sulfate suspension

specific activity

500,000-1,000,000 units/mg protein

mol wt

94 kDa

storage temp.

2-8°C

Quality Level

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Analysis Note

通过缩二脲法测定的蛋白质。

Application

大豆酶将使用花生四烯酸作为底物,以亚油酸作为底物的活性约为 15%;花生四烯酸氧化的产物是 12-或 15-过氧化氢花生四烯酸(12-HPETE 或 15-HPETE)。
来自Glycine max (大豆)的脂氧酶(也称脂肪氧合酶)已被用于修饰从人血浆中分离的低密度脂蛋白。

Biochem/physiol Actions

催化含有顺式顺式-1,4-戊二烯结构的脂质的氢过氧化。

Other Notes

一个酶活性单位是指在pH 9.0、25°C 下,在3.0 ml体积(1 cm光程)中使用亚油酸作为底物时,每分钟使A234 增加0.001所需的酶量。一个A234 单位相当于μmole亚油酸的氧化。

Physical form

悬浮于2.3 M(NH42SO4溶液中,pH约为6.0

Preparation Note

通过离子交换色谱和疏水相互作用色谱法制备。

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

动植物源性产品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Aldana M Ramirez et al.
Lipids, 48(10), 1005-1015 (2013-07-31)
The lipid precursor alcohols of pyrethrins-jasmolone, pyrethrolone and cinerolone-have been proposed as sharing parts of the oxylipin pathway with jasmonic acid. This implies that one of the first committed steps of pyrethrin biosynthesis is catalyzed by a lipoxygenase, catalyzing the
E Wieland et al.
Proceedings of the National Academy of Sciences of the United States of America, 90(13), 5929-5933 (1993-07-01)
Oxidative modification of low density lipoprotein is believed to be an important pathway by which the lipoprotein becomes atherogenic. The in vitro systems for oxidative modification of low density lipoprotein thus far described all appear to depend upon the presence
Nisreen Faizo et al.
Foods (Basel, Switzerland), 10(2) (2021-02-07)
Lipid peroxides (LOOHs) abound in processed food and have been implicated in the pathology of diverse diseases including gut, cardiovascular, and cancer diseases. Recently, RNA Sequencing (RNA-seq) has been widely used to profile gene expression. To characterize gene expression and
P Harduin et al.
Journal of lipid research, 36(5), 919-930 (1995-05-01)
We studied the effect of in vitro moderate oxidation on low density lipoprotein (LDL) conformation and metabolism. LDL was modified with either copper ions or phospholipase A2 plus lipoxygenase and, in both cases, mild oxidative conditions were used. The resulting
Marc P Baggelaar et al.
Bioorganic & medicinal chemistry, 21(17), 5271-5274 (2013-07-23)
A catalytic asymmetric synthesis of (S)-(-)-zearalenone is reported using asymmetric allylic alkylation for the introduction of the stereocenter. (S)-(-)-Zearalenone turned out to be a novel lipoxygenase inhibitor.

商品

Instructions for working with enzymes supplied as ammonium sulfate suspensions

以硫酸铵悬浮液形式提供的酶的使用指南

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