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L6876

Lysozyme 来源于鸡蛋白

Name: Lysozyme 来源于鸡蛋白
Brand: SIGMA

powder or granules, ≥39,000 units/mg protein

别名:

粘肽N-乙酰胞壁质水解酶, 胞壁质酶

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关于此项目

化学文摘社编号:

12650-88-3

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

235-747-3

MDL number:

MFCD00131557

EC 号:

3.2.1.17 (BRENDA, IUBMB)

Specific activity:

≥39,000 units/mg protein

Biological source:

chicken egg white

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biological source

chicken egg white

Quality Level

200

form

powder or granules

specific activity

≥39,000 units/mg protein

mol wt

single-chain 14.3 kDa

technique(s)

cell based assay: suitable

suitability

suitable for cell lysis

UniProt accession no.

P00698

application(s)

cell analysis

storage temp.

room temp

Gene Information

chicken ... LYZ(396218)

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Application

来自鸡蛋清的溶菌酶已用于从细菌细胞中提取基因组DNA。它已被用作MALDI-TOF（基质辅助激光解吸电离-飞行时间）质谱分析的一种外部标准品。

酶可分解细菌的细胞壁；用于制备原生质体。

Biochem/physiol Actions

溶菌酶可水解肽聚糖中N-乙酰胞壁酸与N-乙酰-D-氨基葡萄糖残基之间以及壳糊精中N-乙酰-D-氨基葡萄糖残基之间的β（1→4）连接。革兰氏阳性细胞对这种水解非常敏感，因为它们的细胞壁中的肽聚糖比例很高。革兰氏阴性菌就对这种水解没那么敏感，因为它们有外膜并且肽聚糖的比例较低。然而，这些细胞可能在细胞外膜中螯合金属离子的EDTA存在的情况下水解。

该酶在广泛的pH范围（6.0至9.0）内具有活性。pH值为6.2时，可在更广的离子强度范围（0.02至0.100 M）观察到最大活性，而pH值为9.2时为0.01至0.06 M。

溶菌酶可水解肽聚糖中N-乙酰胞壁酸与N-乙酰-D-氨基葡萄糖残基之间以及壳糊精中N-乙酰-D-氨基葡萄糖残基之间的β（1→4）连接。革兰氏阳性细胞对这种水解非常敏感，因为它们的细胞壁中的肽聚糖比例很高。

Features and Benefits

Lysozyme 是一种多功能酶，具有独特性质，适用于各种应用，包括细菌细胞壁分解、基因组DNA提取和质量分析的外部标准。它在广泛的pH值范围内性能可靠、活跃，可采用各种包装尺寸。

Other Notes

查看更多关于复合糖分析用酶的信息，请访问 www.sigma-aldrich.com/enzymeexplorer

酶用缓冲液悬浮后，在pH 6.2、25 °C下，1单位每分钟可释放0.6 μg溶壁微球菌（浊度法检测，600nm）。

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

低风险生物材料

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历史批次信息供参考:

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商品

HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4

For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein

实验方案

Enzymatic Assay of Lysozyme (EC 3.2.1.17)

This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

溶菌酶的酶学测定（EC 3.2.1.17）

该酶速率测定可用于溶菌酶产物。它不适用于在测定在琼脂糖上的重组型或不溶性溶菌酶。

全球贸易项目编号

货号	GTIN
L6876-5G	04061835573523
L6876-25G	04061835516148
L6876-100G	04061835512706
L6876-1G	04061835573516
L6876-10G	04061835521920

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