L7391
Anti-LIS1 antibody, Mouse monoclonal
clone LIS1-338, purified from hybridoma cell culture
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关于此项目
Conjugate:
unconjugated
Clone:
LIS1-338, monoclonal
Application:
ARR, IP, WB
Citations:
15
InChI key
ZQTAMPRZFOOEEP-AVQMFFATSA-N
SMILES string
CCCCC\C=C\C\C=C\CCCCCCCC(=O)OCC(O)COP(O)(O)=O
InChI
1S/C21H39O7P/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-21(23)27-18-20(22)19-28-29(24,25)26/h6-7,9-10,20,22H,2-5,8,11-19H2,1H3,(H2,24,25,26)/b7-6+,10-9+
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
LIS1-338, monoclonal
form
buffered aqueous solution
mol wt
antigen 46 kDa
species reactivity
zebrafish, chicken, rat, human, bovine, mouse
concentration
~2 mg/mL
technique(s)
immunoprecipitation (IP): suitable, microarray: suitable, western blot: 0.5-1 μg/mL using a rat brain cytosol preparation
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PAFAH1B1(5048)
mouse ... Pafah1b1(18472)
rat ... Pafah1b1(83572)
General description
Monoclonal Anti-LIS1 (mouse IgG1 isotype) is derived from the LIS1-338 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a recombinant human LIS1 protein. The LIS1 protein (46 kDa) contains seven WD (tryptophan-aspartic acid) repeats, a motif shared by at least 140 known proteins.
The gene PAFAH1B1 (platelet activating factor acetylhydrolase 1b regulatory subunit 1) encodes a protein named LIS1 (lissencephaly-1) that forms the α subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase (PAFAH). The gene is mapped to human chromosome 17p13.3.
Immunogen
recombinant human LIS1 protein.
Application
Monoclonal Anti-LIS1 antibody produced in mouse has been used in protein pulldown assay and immunofluorescence.
Biochem/physiol Actions
By immunoblotting, the antibody prefers the phosphorylated form of the LIS1 molecule (46 kDa).
LIS1 is involved in cell regulation, including the β subunits of G-proteins (Gβ). This protein motif is likely to mediate protein-protein interactions. LIS1 is a protein that is highly conserved during evolution; bovine, mouse, and chicken proteins exceed 99% similarity.
The heterotrimeric enzyme, platelet-activating factor acteylhydrolase (PAFAH), catalyzes the cleavage of acetyl group at the SN-2 position of platelet-activating factor. The α subunit encoded by the gene PAFAH1B1 (platelet activating factor acetylhydrolase 1b regulatory subunit 1) interacts with tubulin and affects microtubule dynamics. Mutations in this gene have been linked to Miller–Dieker lissencephaly, a human brain malformation characterized by a smooth cerebral surface and a disordered organization of the cortical layers resulting from a defect in neuronal migration.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
此项目有
Richard J McKenney et al.
The Journal of biological chemistry, 286(45), 39615-39622 (2011-09-14)
Cytoplasmic dynein is responsible for a wide range of cellular roles. How this single motor protein performs so many functions has remained a major outstanding question for many years. Part of the answer is thought to lie in the diversity
Armen J Moughamian et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(32), 13190-13203 (2013-08-09)
Long-range retrograde axonal transport in neurons is driven exclusively by the microtubule motor cytoplasmic dynein. The efficient initiation of dynein-mediated transport from the distal axon is critical for normal neuronal function, and neurodegenerative disease-associated mutations have been shown to specifically
Shusheng Wang et al.
The Journal of biological chemistry, 286(1), 587-593 (2010-11-09)
The nuclear distribution protein E (NudE) and nuclear distribution protein E-like (Nudel or Ndel1) interact with both lissencephaly 1 (Lis1) and dynein. These interactions are thought to be essential for dynein function. Previous studies have shown that the highly conserved
Interaction between LIS1 and doublecortin, two lissencephaly gene products
Caspi M, et al.
Human Molecular Genetics, 9(15), 2205-2213 (2000)
Isolation of a Miller-Dieker lissencephaly gene containing G protein beta-subunit-like repeats.
Reiner O
Nature, 364, 717-721 (1993)
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