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L7525

Sigma-Aldrich

L-Lactic Dehydrogenase 来源于猪心脏

ammonium sulfate suspension, ≥200 units/mg protein

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别名:
(S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD
CAS号:
9001-60-9
EC 号:
1.1.1.27 (BRENDA, IUBMB)
EC 号:
232-617-8
MDL编号:
MFCD00131466
NACRES:
NA.54

生物来源

Porcine heart

质量水平

200

形式

ammonium sulfate suspension

比活

≥200 units/mg protein

UniProt登记号

P00339

异质活性

glutamic-oxalacetic transaminase ≤0.1%
malic dehydrogenase, glutamic-pyruvic transaminase and pyruvate kinase ≤0.06%

储存温度

2-8°C

基因信息

pig ... LDHA(407245)

生化/生理作用

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

单位定义

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

外形

Suspension in ammonium sulfate and 0.1 M potassium phosphate, pH 7.0

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

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Joe T Sharick et al.
Scientific reports, 8(1), 5456-5456 (2018-04-05)
While NAD(P)H fluorescence lifetime imaging (FLIM) can detect changes in flux through the TCA cycle and electron transport chain (ETC), it remains unclear whether NAD(P)H FLIM is sensitive to other potential fates of glucose. Glucose carbon can be diverted from
M J Adams et al.
Proceedings of the National Academy of Sciences of the United States of America, 70(7), 1968-1972 (1973-07-01)
The binding of coenzyme and substrate are considered in relation to the known primary and tertiary structure of lactate dehydrogenase (EC 1.1.1.27). The adenine binds in a hydrophobic crevice, and the two coenzyme phosphates are oriented by interactions with the
H P Schär et al.
Hoppe-Seyler's Zeitschrift fur physiologische Chemie, 360(7), 795-807 (1979-07-01)
Lactate dehydrogenases from thermophilic bacilli (Bacillus stearothermophilus, Bacillus caldotenax) and from mesophilic bacilli (Bacillus X1, Bacillus subtilis) have been isolated by a two-step purification procedure. Only one type (LDH-P4) composed of four identical subunits (Mr 34 000 or 36 000)
Brian M Peters et al.
Infection and immunity, 82(2), 532-543 (2014-01-31)
Vulvovaginal candidiasis, caused primarily by Candida albicans, presents significant health issues for women of childbearing age. As a polymorphic fungus, the ability of C. albicans to switch between yeast and hyphal morphologies is considered its central virulence attribute. Armed with
José A Jara et al.
Journal of medicinal chemistry, 57(6), 2440-2454 (2014-02-27)
Tumor cells principally exhibit increased mitochondrial transmembrane potential (ΔΨ(m)) and altered metabolic pathways. The therapeutic targeting and delivery of anticancer drugs to the mitochondria might improve treatment efficacy. Gallic acid exhibits a variety of biological activities, and its ester derivatives
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实验方案

Enzymatic Assay of L-Lactic Dehydrogenase (EC 1.1.1.27)

This procedure applies to all products from heart muscle that have a specification for L-Lactic Dehydrogenase activity.

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