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L7658

Sigma-Aldrich

LB 肉汤 (Lennox)

EZMix® powder microbial growth medium

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别名:
Lennox broth
NACRES:
NA.85

等级

for molecular biology

质量水平

200

描述

quick-dissolve, dust-free formulation

无菌性

non-sterile

形式

powder

技术

microbiological culture: suitable

pH值（酸碱度）

6.8-7.2(2.5% solution)

应用

agriculture
food and beverages
microbiology

储存温度

room temp

适用性

nonselective for Escherichia coli
nonselective for coliforms

相关类别

一般描述

Lennox LB是一种被高度引用的微生物生长培养基，适用于培养大肠杆菌。这种营养丰富的微生物肉汤采用低盐配方，含有多肽、氨基酸、水溶性维生素和碳水化合物。

应用

适用于大肠杆菌菌株的非选择性培养，用于克隆、DNA质粒生产和重组蛋白生产。当加入适当的抗生素时，也适用于选择性培养，包括需要低盐条件的抗生素，如博莱霉素®。

特点和优势

Lennox LB EZMix®粉末提供：
  • 无尘颗粒，便于安全操作和更快混合
  • 便捷式小包装，无需称重步骤
  • 使用大包装可轻松放大规模
  • 标准配方

组分

*请注意，某些产品规格以液体形式提供。本品为粉末形式，并且这些规格反映了最终的重悬体积。

10g/L 胰蛋白胨
5 g/L 酵母提取物
5 g/L NaCl
0.6 g/L 惰性粘合剂（仅限EZMix配方）

制备说明

1.将20.6 g悬浮于1L蒸馏水中。
2.在121℃下高压蒸汽灭菌15分钟。
制备LB肉汤：加入1g 葡萄糖，再按上述制备说明操作。
制备Enquist和Sternberg培养基：高压蒸汽灭菌后，无菌操作下加入10 ml无菌1M硫酸镁。

重悬

搅拌，使20.6g粉末悬浮于1L水中。在121°C下高温灭菌15分钟以灭菌。先冷却再添加其他成分，如抗生素（如果需要）。

法律信息

EZMix is a registered trademark of Merck KGaA, Darmstadt, Germany
Zeocin is a registered trademark of Cayla Sarl

相关产品

产品编号
说明
价格

L7275

LB肉汤（Lennox）, Tablet microbial growth medium

L7025

含琼脂的 LB 肉汤 (Lennox), Tablet microbial growth medium

L7533

LB Broth with agar (Lennox), EZMix® powder microbial growth medium

L3022

LB 肉汤（Lennox）, Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.

L2897

LB 琼脂, Highly-referenced microbial growth powder medium with Agar, low salt, suitable for salt-sensitive E. coli culture.

L5542

LB Agar Plates, pre-poured LB agar plates for E. coli

CMC0001

SIG10 化学感受态细胞, for protein expression and DNA plasmid production

CMC0007

SIG10 5a化学感受态细胞, for DNA plasmid production

CMC0014

BL21（DE3）化学感受态细胞, for protein expression

CMC0017

过表达 ™ C41 (DE3) 化学感受态细胞, for the highest protein expression

储存分类代码

11 - Combustible Solids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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示例

T1503
货号
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25G
包装规格/数量

其它示例：

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

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  • 如果您查询到的批号为 TO09019TO 等，请输入去除前两位字母的批号：09019TO。

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部分情况下，可能未在线提供COA。如果搜索不到COA，可在线索取。

索取COA

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What are the differences among the Luria, Lennox and Miller LB formulations?

    LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose.  Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version).  For some applications, even lower salt is required (Luria's low salt version). 

  6. What is the difference among the many LB - Lennox products?

    LB - Lennox is available in many types to suit your needs.  The different product formats include powder, EZMix™ powder, and tablet form.  Each of these forms are available with and without agar for easy LB-agar plate preparation.L3022 (LB Lennox powder)L2897 (LB Lennox powder with agar)L7275 (LB Lennox tablets)L7025 (LB Lennox tablets with agar)L7658 (LB Lennox EZMix(TM) powder)L7533 (LB Lennox EZMix(TM) powder with agar)L5542 (LB Lennox pre-poured agar plates)

  7. Which bacterial culture medium is the best choice for my application?

    Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production.  The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.

  8. Will adding magnesium to the culture medium increase cell density?

    In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities.  You may need to also increase the shaking speed of the incubator. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Jennifer White et al.
Protein science : a publication of the Protein Society, 13(9), 2406-2415 (2004-08-24)
Decay-accelerating factor (DAF, CD55) is a glycophosphatidyl inositol-anchored glycoprotein that regulates the activity of C3 and C5 convertases. In addition to understanding the mechanism of complement inhibition by DAF through structural studies, there is also an interest in the possible
Michael C Konopka et al.
Journal of bacteriology, 188(17), 6115-6123 (2006-08-23)
The first in vivo measurements of a protein diffusion coefficient versus cytoplasmic biopolymer volume fraction are presented. Fluorescence recovery after photobleaching yields the effective diffusion coefficient on a 1-mum-length scale of green fluorescent protein within the cytoplasm of Escherichia coli
Katherine A Kantardjieff et al.
Acta crystallographica. Section D, Biological crystallography, 60(Pt 5), 895-902 (2004-04-23)
The Mycobacterium tuberculosis rmlC gene encodes dTDP-4-keto-6-deoxyglucose epimerase, the third enzyme in the M. tuberculosis dTDP-L-rhamnose pathway which is essential for mycobacterial cell-wall synthesis. Because it is structurally unique, highly substrate-specific and does not require a cofactor, RmlC is considered
Alexis Kaushansky et al.
Nature protocols, 5(4), 773-790 (2010-04-03)
Protein microarrays provide an efficient way to identify and quantify protein-protein interactions in high throughput. One drawback of this technique is that proteins show a broad range of physicochemical properties and are often difficult to produce recombinantly. To circumvent these
Feng Xu et al.
PloS one, 6(4), e19344-e19344 (2011-05-10)
Decellularization and cellularization of organs have emerged as disruptive methods in tissue engineering and regenerative medicine. Porous hydrogel scaffolds have widespread applications in tissue engineering, regenerative medicine and drug discovery as viable tissue mimics. However, the existing hydrogel fabrication techniques
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实验方案

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

微生物生长实验方案

在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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