grade
Molecular Biology
Quality Level
description
quick-dissolve, dust-free formulation
sterility
non-sterile
form
powder
technique(s)
microbiological culture: suitable
pH
6.8-7.2(2.5% solution)
application(s)
agriculture
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
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General description
Lennox LB是一种被高度引用的微生物生长培养基，适用于培养大肠杆菌。这种营养丰富的微生物肉汤采用低盐配方，含有多肽、氨基酸、水溶性维生素和碳水化合物。
Application
适用于大肠杆菌菌株的非选择性培养，用于克隆、DNA质粒生产和重组蛋白生产。当加入适当的抗生素时，也适用于选择性培养，包括需要低盐条件的抗生素，如博莱霉素®。
Features and Benefits
Lennox LB EZMix粉末提供：
- 无尘颗粒，便于安全操作和更快混合
- 便捷式小包装，无需称重步骤
- 使用大包装可轻松放大规模
- 标准配方
Preparation Note
1.将20.6 g悬浮于1L蒸馏水中。
2.在121℃下高压蒸汽灭菌15分钟。
制备LB肉汤：加入1g 葡萄糖，再按上述制备说明操作。
制备Enquist和Sternberg培养基：高压蒸汽灭菌后，无菌操作下加入10 ml无菌1M硫酸镁。
2.在121℃下高压蒸汽灭菌15分钟。
制备LB肉汤：加入1g 葡萄糖，再按上述制备说明操作。
制备Enquist和Sternberg培养基：高压蒸汽灭菌后，无菌操作下加入10 ml无菌1M硫酸镁。
搅拌，使20.6g粉末悬浮于1L水中。在121°C下高温灭菌15分钟以灭菌。先冷却再添加其他成分，如抗生素（如果需要）。
Other Notes
*请注意，某些产品规格以液体形式提供。本品为粉末形式，并且这些规格反映了最终的重悬体积。
10g/L 胰蛋白胨
5 g/L 酵母提取物
5 g/L NaCl
0.6 g/L 惰性粘合剂（仅限EZMix配方）
10g/L 胰蛋白胨
5 g/L 酵母提取物
5 g/L NaCl
0.6 g/L 惰性粘合剂（仅限EZMix配方）
Legal Information
Zeocin is a registered trademark of Cayla Sarl
存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
监管及禁止进口产品
此项目有
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实验方案
General protocols for growth of competent cells in microbial medium.
在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| L7658-2X5L | 04061833965658 |
| L7658-1KG | 04061835574315 |
| L7658-6X500ML | 04061835513819 |
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