biological source
Arachis hypogaea (peanut)
Quality Level
conjugate
peroxidase conjugate
form
lyophilized powder
peroxidase activity
≥20 units/mg protein
technique(s)
western blot: suitable
storage temp.
−20°C
Application
通用 Western 印迹法方案：
- 糖蛋白样本量：500 ng
- 凝集素浓度：0.1 μg/mL
- 每个泳道加入500 ng糖蛋白样品
- 运行 4-20％Bis-Tris SDS 页面凝胶
- 将凝胶转移至 PVDF 膜上
- 用 RTA 缓冲液（R0278 Sigma）在室温下封闭膜 1 小时
- 在室温下用 RIPA 缓冲液将 HRP 凝集素以 0.1ug / ml 孵育 2 小时
- 用 25ml RIPA 缓冲液洗涤膜 5×5 分钟
- 使用化学发光底物（CPS1-120）进行检测
Biochem/physiol Actions
花生凝集素不会凝集正常的人类红细胞，但会强烈凝集神经氨酸酶处理的红细胞。花生凝集素具有与人血清中的抗 T 抗体相似的有效抗 T 活性。凝集素可用于区分人淋巴细胞亚群。
Physical form
含柠檬酸钠
Preparation Note
采用改进的已发表方法，通过过氧化物酶 (P8375) 制备，有利于低分子量偶联物。通过亲和层析缀合后再纯化。
Other Notes
在 20°C、pH 值为 6.0 条件下，1 个单位的邻苯三酚在 20 s 内会形成 1 mg 红桔酚。
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存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| L7759-1MG | 04061835557608 |
| L7759-250UG | 04061833965672 |