跳转至内容
Merck
CN

L7759

花生的凝集素

peroxidase conjugate, lyophilized powder

别名:

PNA, 花生凝集素

登录 查看组织和合同定价。

选择尺寸

关于此项目

NACRES:
NA.54
UNSPSC Code:
12352202

主要文件

查看所有文档
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

产品名称

花生的凝集素, peroxidase conjugate, lyophilized powder

biological source

Arachis hypogaea (peanut)

conjugate

peroxidase conjugate

form

lyophilized powder

peroxidase activity

≥20 units/mg protein

technique(s)

western blot: suitable

storage temp.

−20°C

Quality Level

200

正在寻找类似产品? 访问 产品对比指南

相关类别

Application

通用 Western 印迹法方案：
  • 糖蛋白样本量：500 ng
  • 凝集素浓度：0.1 μg/mL

  1. 每个泳道加入500 ng糖蛋白样品
  2. 运行 4-20％Bis-Tris SDS 页面凝胶
  3. 将凝胶转移至 PVDF 膜上
  4. 用 RTA 缓冲液（R0278 Sigma）在室温下封闭膜 1 小时
  5. 在室温下用 RIPA 缓冲液将 HRP 凝集素以 0.1ug / ml 孵育 2 小时
  6. 用 25ml RIPA 缓冲液洗涤膜 5×5 分钟
  7. 使用化学发光底物（CPS1-120）进行检测

Biochem/physiol Actions

花生凝集素不会凝集正常的人类红细胞，但会强烈凝集神经氨酸酶处理的红细胞。花生凝集素具有与人血清中的抗 T 抗体相似的有效抗 T 活性。凝集素可用于区分人淋巴细胞亚群。

Other Notes

在 20°C、pH 值为 6.0 条件下，1 个单位的邻苯三酚在 20 s 内会形成 1 mg 红桔酚。

Physical form

含柠檬酸钠

Preparation Note

采用改进的已发表方法，通过过氧化物酶 (P8375) 制备，有利于低分子量偶联物。通过亲和层析缀合后再纯化。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

动植物源性产品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000438912
0000438912
0000438911
0000438911
0000404689

没有发现合适的版本？

如果您需要特殊版本，可通过批号或批次号查找具体证书。

搜索COA

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. Is the peanut lectin (Lectin from Arachis hypogaea) a glycoprotein?  If so, what is the carbohydrate content?

    Peanut lectin is not a glycoprotein. Although peanut lectin binds specifically to a particular carbohydrate (beta-gal(1-->3)galNac) in other molecules, the lectin itself does not contain any carbohydrate.  Please see page 231 of the following paper: R. Banerjee, et al., "Crystal structure of peanut lectin, a protein with an unusual quaternary structure".  Proc. Nat. Acad. Sci., USA, 91, 227-231 (1994).

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Matthew R Kudelka et al.
Nature methods, 13(1), 81-86 (2015-12-01)
Protein O-glycosylation has key roles in many biological processes, but the repertoire of O-glycans synthesized by cells is difficult to determine. Here we describe an approach termed Cellular O-Glycome Reporter/Amplification (CORA), a sensitive method used to amplify and profile mucin-type
Timothy J Powell et al.
The Journal of general virology, 100(3), 431-445 (2019-02-05)
A non-replicating form of pseudotyped influenza virus, inactivated by suppression of the haemagglutinin signal sequence (S-FLU), can act as a broadly protective vaccine. S-FLU can infect for a single round only, and induces heterotypic protection predominantly through activation of cross-reactive
J-Z Chan et al.
Andrologia, 34(6), 379-383 (2002-12-11)
Lectins are useful for staining the acrosome, which is a pre-requisite for the assessment of acrosome reaction in vitro. We tested wheat germ agglutinin, peanut agglutinin and pisum sativum agglutinin. The determination of the categories of normal and abnormal spermatozoa
Anna Iervolino et al.
PloS one, 10(3), e0119142-e0119142 (2015-03-24)
Dicer is a crucial enzyme for the maturation of miRNAs. Mutations in the Dicer gene are highly associated with Pleuro Pulmonary Blastoma-Family Dysplasia Syndrome (PPB-FDS, OMIM 601200), recently proposed to be renamed Dicer syndrome. Aside from the pulmonary phenotype (blastoma)
Ting-Hsuan Chen et al.
Methods in molecular biology (Clifton, N.J.), 2248, 139-153 (2020-11-14)
Virus-like particle (VLP) technology is an alternative platform for developing vaccines to combat seasonal and pandemic influenza. Influenza VLPs are non-infectious nanoparticles that can elicit effective vaccine immunogenicity in hosts. B-cell-activating factor (BAFF, or BLyS) and a proliferation-inducing ligand (APRIL)
查看所有相关文献

相关内容

Product Information Sheet - L7759

Product Information Sheet

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持