Merck
CN

L7773

Sigma-Aldrich

Lysozyme 来源于鸡蛋白

aseptically filled

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别名:
粘肽N-乙酰胞壁质水解酶, 胞壁质酶
CAS号:
EC 号:
MDL编号:
NACRES:
NA.54

无菌性

aseptically filled

质量水平

形式

powder

enzyme activity

≥40000 units/mg protein

分子量

single-chain 14.3 kDa

UniProt登记号

储存温度

−20°C

基因信息

chicken ... LYZ(396218)

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应用

酶可分解细菌的细胞壁;用于制备原生质体。

生化/生理作用

溶菌酶可水解肽聚糖中N-乙酰胞壁酸与N-乙酰-D-氨基葡萄糖残基之间以及壳糊精中N-乙酰-D-氨基葡萄糖残基之间的β(1→4)连接。 革兰氏阳性细胞对这种水解非常敏感,因为它们的细胞壁中的肽聚糖比例很高。革兰氏阴性菌就对这种水解没那么敏感,因为它们有外膜并且肽聚糖的比例较低。然而,这些细胞可能在细胞外膜中螯合金属离子的EDTA存在的情况下水解。

该酶在广泛的pH范围(6.0至9.0)内具有活性。pH值为6.2时,可在更广的离子强度范围(0.02至0.100 M)观察到最大活性,而pH值为9.2时为0.01至0.06 M。

单位定义

一个酶活性单位是指在pH 6.24、25 °C条件下,以溶壁微球菌( Micrococcus lysodeikticus )悬浮液作为底物,在2.6 mL的反应混合物中(1 cm光路),每分钟产生0.001的 ΔA450 所需的酶量。

制备说明

使用L6876制备

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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Dennis J Orton et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 921-922, 75-80 (2013-02-26)
As an alternative to direct UV absorbance measurements, estimation of total protein concentration is typically conducted through colorimetric reagent assays. However, for protein-limited applications, the proportion of the sample sacrificed to the assay becomes increasingly significant. This work demonstrates a
Vytautas Gapsys et al.
Biophysical journal, 104(1), 196-207 (2013-01-22)
Analysis of the internal dynamics of a biological molecule requires the successful removal of overall translation and rotation. Particularly for flexible or intrinsically disordered peptides, this is a challenging task due to the absence of a well-defined reference structure that
Robert D Turner et al.
Nature communications, 4, 1496-1496 (2013-02-21)
Cellular integrity and morphology of most bacteria is maintained by cell wall peptidoglycan, the target of antibiotics essential in modern healthcare. It consists of glycan strands, cross-linked by peptides, whose arrangement determines cell shape, prevents lysis due to turgor pressure
Mahdieh Mansouri et al.
Molecules (Basel, Switzerland), 18(1), 789-813 (2013-01-25)
The interactions between cyclophosphamide (CYC) and lysozyme (LYZ) in the presence of different cyclodextrins (CDs) were investigated by UV absorption, fluorescence spectroscopy, circular dichroism (CD), and molecular modeling techniques under imitated physiological conditions. The UV absorption results showed the formation
Anne Derbise et al.
The Journal of infectious diseases, 207(10), 1535-1543 (2013-02-14)
Yersinia pestis (the plague bacillus) and its ancestor, Yersinia pseudotuberculosis (which causes self-limited bowel disease), encode putative homologues of the periplasmic lysozyme inhibitor Ivy and the membrane-bound lysozyme inhibitor MliC. The involvement of both inhibitors in virulence remains subject to

实验方案

This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

该酶速率测定可用于溶菌酶产物。它不适用于在测定在琼脂糖上的重组型或不溶性溶菌酶。

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