L8146
来自 荆豆（gorse、furze）的凝集素
peroxidase conjugate, lyophilized powder
别名:
荆豆凝集素, UEA-I
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关于此项目
NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder
Assay:
70.00-90.00% (Lowry)
Biological source:
Ulex europaeus
biological source
Ulex europaeus
conjugate
peroxidase conjugate
assay
70.00-90.00% (Lowry)
form
lyophilized powder
potency
<40 μg/mL agglutination activity
peroxidase activity
≥15 units/mg protein
composition
Protein, ~80% Lowry
technique(s)
western blot: suitable
storage temp.
2-8°C
Quality Level
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Application
通用蛋白质印迹方案：
- 糖蛋白样品规格：500ng
- 凝集素浓度： 0.1ug/ml
- 每条泳道上样500 ng糖蛋白样品
- 进行4-20% Bis-Tris SDS page凝胶
- 转凝胶至PVDF膜上
- 用于RIPA缓冲液（R0278 Sigma）在室温对膜进行封闭1小时
- 在室温用RIPA缓冲液以0.1 ug/ml孵育HRP凝集素2小时
- 用25ml RIPA缓冲液洗膜 5 x 5分钟
- 用化学发光底物（CPS1-120）进行检测
Biochem/physiol Actions
荆豆凝集素具有抗H血型特异性。凝集素有两种类型： UEA I 对 L-岩藻糖具有亲和力，而 UEA II 对 N,N′-二乙酰壳二糖具有亲和力。最初认为UEA I摩尔量为170,000道尔顿。 后来的报道表明，UEA I可以在中性和碱性pH下形成聚集体，正确的摩尔量是68,000道尔顿。
Packaging
包装尺寸基于蛋白质含量。
Physical form
含有柠檬酸钠缓冲盐
Preparation Note
由过氧化物酶，VI型（P8375），使用Avrameas和Ternyck的两步戊二醛方法制备。
偶联后通过亲和层析重新纯化。
偶联后通过亲和层析重新纯化。
Analysis Note
凝集活性以μg/ml表示，由磷酸盐缓冲盐水（pH 6.8）连续稀释1 mg/ml溶液而测得。该活性是指25℃孵育1小时后使2％人O型血红细胞悬浮液凝集的最低浓度。
Other Notes
一单位在20℃、pH 6.0条件下，在20秒内从邻苯三酚形成1.0 mg的红棓酚。
由亲和纯化的凝集素制备缀合物。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
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