form
lyophilized powder
potency
<20 μg per mL agglutination activity
composition
Protein, ≥90% Lowry
impurities
salt, essentially free
storage temp.
2-8°C
Quality Level
正在寻找类似产品? 访问 产品对比指南
General description
从 Galanthus nivalis （雪花莲）提取的凝集素是甘露糖结合凝集素，为四聚体，分子量12 kDa。它属于石蒜科（Amaryllidacea）。它对糖类 α-1,3-或1,6-连接的 D-甘露糖有特异性。雪花莲凝集素对昆虫有毒。
Application
从 Galanthus nivalis （雪花莲）提取的凝集素，用于：
- 包被ELISA板
- 作为逆转录酶抑制剂
- 酶联免疫吸附测定，检测人和猿猴免疫缺陷病毒的包膜糖蛋白
Biochem/physiol Actions
GNL 凝集兔红细胞；人红细胞不凝集。GNL 糖特异性指向末端糖结合物的 α-D -甘露糖残基的非还原端。
从 Galanthus nivalis （雪花莲）提取的凝集素可抵抗害虫，用于转基因植物。雪花莲凝集作为载体，转运融合蛋白至血淋巴，在其穿过目标物种肠道上皮细胞时，表现出杀虫作用。
Preparation Note
根据 Kaku 通过亲和色谱法纯化。
Analysis Note
凝集活性以 μg/ml 表示，在磷酸盐缓冲盐水 (pH 7.2) 中连续稀释 1 mg/ml 溶液，测定。该活性是在 25℃ 下孵育 1h 后凝集 2% 兔红细胞悬液的最低浓度。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
监管及禁止进口产品
此项目有
Comparison of serological tests for the diagnosis of feline immunodeficiency virus infection of cats
Sibille P, et al.
Veterinary Microbiology, 45(2-3), 25-267 (1995)
A fusion protein containing a lepidopteran-specific toxin from the South Indian red scorpion (Mesobuthus tamulus) and snowdrop lectin shows oral toxicity to target insects
Trung N, et al
BMC biotechnology, 6, 18-18 (2006)
Jacklyn Johnson et al.
Journal of virology, 94(9) (2020-02-28)
Semen is the primary transmission vehicle for various pathogenic viruses. Initial steps of transmission, including cell attachment and entry, likely occur in the presence of semen. However, the unstable nature of human seminal plasma and its toxic effects on cells
Snowdrop lectin.
H Kaku et al.
Methods in enzymology, 179, 327-331 (1989-01-01)
Expression of snowdrop lectin (GNA) in transgenic rice plants confers resistance to rice brown planthopper
Rao KV, et al.
The Plant Journal, 15, 469-477 (1998)
相关内容
Instructions
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持