biological source
Phaseolus vulgaris
Quality Level
form
lyophilized powder
potency
≤62.5 μg per mL agglutination activity, ≤10 μg/mL mitogenic activity
impurities
salt, free
storage temp.
2-8°C
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General description
PHA是碳水化合物结合蛋白。已鉴定出几种类型的PHA，主要存在于豆科植物中。
植物血凝素（PHA）是在植物中发现的一种凝集素（特别是在豆科植物中）。它来自于 菜豆（Phaseolus vulgaris） （红芸豆），并可作为人类白细胞培养物中的有丝分裂原。
Application
可用作抗原，测定 体内 T细胞介导的免疫应答。
来自 菜豆（Phaseolus vulgaris） （红芸豆）的凝集素可用于植物血凝素（PHA）检测 ，以及评估细胞和体液免疫力。
Biochem/physiol Actions
PHA由两种分子种类组成，即具有低促有丝分裂活性和高红细胞凝集素活性的红细胞凝集素（PHA-E），和具有高促有丝分裂和白细胞凝集活性但具有非常低的红细胞凝集活性的白细胞凝集素（PHA-L）。
PHA-E不具有血型特异性，但其凝集活性可被某些低聚糖抑制。 在分离和纯化红细胞凝集素和白细胞凝集素之前，PHA-P是PHA的蛋白质形式。 PHA-M是粘蛋白形式。 结合物由相应的纯化凝集素进行制备。
PHA-E不具有血型特异性，但其凝集活性可被某些低聚糖抑制。 在分离和纯化红细胞凝集素和白细胞凝集素之前，PHA-P是PHA的蛋白质形式。 PHA-M是粘蛋白形式。 结合物由相应的纯化凝集素进行制备。
Analysis Note
凝集活性以mg/ml表示，并由1mg/ml溶液的连续稀释液进行测定。该活性是在25℃温育1小时后，在pH6.8的磷酸盐缓冲盐水中,凝集2％人红细胞悬浮液所需的最低浓度。其促有丝分裂活性可通过在HeLa细胞培养物中掺入BrdU来确定。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Phytohemagglutinin content in fresh kidney bean in China
Sun Y, et al.
International journal of food properties, 22(1), 405-413 (2019)
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A R S Badari Nath et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 134, 143-147 (2014-07-10)
Phytohaemagglutinin (PHA) is a lectin obtained from Phaseolus vulgaris (red kidney beans), that acts as a mitogen in human leucocyte culture and is commercially available from Gibco. This PHA (Gibco) was found to be very expensive, hence other inexpensive sources
Andrew Z Wang et al.
The Journal of experimental biology, 223(Pt 20) (2020-09-13)
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Genetic color polymorphism is widespread in nature. There is an increasing interest in understanding the adaptive value of heritable color variation and trade-off resolution by differently colored individuals. Melanin-based pigmentation is often associated with variation in many different life history
商品
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| L8754-5MG | 04061835557622 |
| L8754-25MG | 04061835557615 |
| L8754-50MG | 04061833966167 |
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