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Merck
CN

L8777

来自美洲商陆(商陆)的凝集素

lyophilized powder, BioReagent, suitable for cell culture

别名:

PWM, 美洲商陆有丝分裂原

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关于此项目

UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
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产品名称

来自美洲商陆(商陆)的凝集素, lyophilized powder, BioReagent, suitable for cell culture

sterility

aseptically filled

product line

BioReagent

form

lyophilized powder

potency

≤0.3 μg/mL mitogenic activity

technique(s)

cell culture | mammalian: suitable

solubility

PBS: 1 mg/mL

storage temp.

2-8°C

Quality Level

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Analysis Note

通过将 BrdU 掺入 HeLa 细胞培养基中测定有丝分裂促进活性。
部分纯化的 TCA 沉淀。
基本不含盐。

Application

来自美洲商陆(商陆)的凝集素用于增殖测定。(使用常规测定,通过在新合成的DNA中掺入 3H 胸苷来测量细胞增殖反应)。

Biochem/physiol Actions

PWM 从商陆根中提取。该蛋白质具有红血球凝集、白血球凝集和促有丝分裂特性。PWM 不具有血型特异性,但具有与 N-乙酰基-β-D-葡糖胺低聚物的亲和性。
凝集素是碳水化合物结合蛋白,对糖部分具有高度特异性。商陆凝集素(PL)是对含有N-乙酰氨基葡糖的糖特异的凝集素,其通过与细胞表面结合来刺激外周淋巴细胞进行有丝分裂。从商陆(美洲商陆)的根中分离出四种类型的凝集素。它们含有同源结构域,但具有不同的分子大小和生物学特性。

General description

凝集素是植物来源的蛋白质,其具有结合某些神经元表面糖蛋白和糖脂的能力。他们的这种能力有助于它们被用作神经解剖学示踪剂,因为它们以逆行或顺行方式被采集和运输。

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

动植物源性产品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Kurt Hanevik et al.
The Journal of infectious diseases, 204(11), 1779-1786 (2011-10-13)
Clinical and epidemiological studies have suggested the development of acquired immunity in individuals previously infected with Giardia lamblia. However, there are no data on the long-term cellular immunity and genotype cross-reactivity. An outbreak of assemblage B giardiasis in a nonendemic
Tomomi Fujii et al.
Acta crystallographica. Section D, Biological crystallography, 60(Pt 4), 665-673 (2004-03-25)
Pokeweed lectin (PL), a lectin specific for N-acetylglucosamine-containing saccharides, stimulates peripheral lymphocytes to undergo mitosis by binding to their cell surfaces. Four types of lectins have been isolated from the roots of pokeweed (Phytolacca americana) and shown to contain homologous
Caroline Leeth et al.
Animals : an open access journal from MDPI, 11(10) (2021-10-24)
Immunological studies in the horse are frequently hampered by lack of environmental control, complicated study design, and ethical concerns when performing high risk studies. The purpose of the current study was to investigate the utility of a xenograft model for
Sharon N Cox et al.
Kidney international, 78(4), 396-407 (2010-05-21)
Immunoglobulin A nephropathy (IgAN) is the most common form of primary glomerulonephritis worldwide. The basic defect lies within the IgA immune system and in peripheral blood leukocytes, rather than local kidney abnormalities. To define the intracellular mechanisms leading to the
A randomized, placebo-controlled phase I study assessing the safety and immunogenicity of a Pseudomonas aeruginosa hybrid outer membrane protein OprF/I vaccine (IC43) in healthy volunteers.
Westritschnig K, Hochreiter R, Wallner G, et al.
Human Vaccines & Immunotherapeutics, 10(1), 170-183 (2014)

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