L8793

Anti-MAP1LC3A antibody

Name: Anti-MAP1LC3A antibody
Brand: SIGMA

rabbit polyclonal

别名:

抗MAP1A/1B轻链3 A, 抗MAP1A/MAP1B LC3 A, 抗MAP1ALC3, 抗MAP1BLC3, 抗MAP1LC3A, 抗MAP1轻链3样蛋白1, 抗微管相关蛋白1轻链3α

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关于此项目

UNSPSC Code:

12352203

NACRES:

NA.41

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IP, WB

Citations:

主要文件

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产品名称

抗LC3A 兔抗, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 16-18 kDa

species reactivity

mouse, human, rat

concentration

~1 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using extracts of human U87 cells, western blot: 0.5-1 μg/mL using whole extracts of rat and mouse brain

UniProt accession no.

Q9H492

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... MAP1LC3A(84557)
mouse ... Map1lc3a(66734)
rat ... Map1lc3a(362245)

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General description

微管相关蛋白 1A/1B 轻链 3 或 MAP1LC3 是一种细胞蛋白，参与自噬体的形成。MAP1LC3 基因具有三种已知的人同工型，即 MAP1LC3A、MAP1LC3B 和 MAP1LC3C。这些同工型对氨基酸饥饿和不同亚细胞定位模式有不同反应。抗 LC3A 抗体通过免疫印迹和免疫沉淀与人和大鼠 LC3A-I（约 18 kDa）和 LC3A-II（约 16 kDa）反应。该抗体对小鼠 LC3A-1（约 18 kDa）也具有特异性。检测小鼠 LC3A-II 可能需要特定的细胞处理和/或长时间暴露。该免疫肽特异性抑制免疫印迹法检测 LC3A 条带。

Immunogen

该合成肽对应于人 LC3A 亚型 α 的氨基酸，通过 C 端半胱氨酸残基与 KLH 偶联。在大鼠和小鼠中的相应序列是相同的。

Application

兔抗LC3A抗体还可用于

免疫组化
免疫染色
蛋白印迹

Biochem/physiol Actions

微管相关蛋白轻链 3-I（LC3-I）被哺乳动物自噬相关 7（Atg7）和 Atg3 修饰为膜结合形式 LC3-II（LC3-磷脂缀合物），分别为 E1- 和 E2- 样酶。LC3 II 的量与自噬小体形成的程度有关。LC3A 也可以作为自噬体标志物。

抗 LC3A 抗体通过免疫印迹和免疫沉淀识别人和大鼠微管相关蛋白轻链 3-A（LC3A-I）和 LC3A-II（分别为〜18 kDa 和〜16 kDa），以及小鼠 LC3A-I（〜18 kDa）。

Physical form

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

Preparation Note

若需连续使用，可在2-8°C下保存最长一个月。若需延长储存时间，可将溶液分装并冷冻。不建议反复冻融或存放在“无霜”冰柜中。如果长期储存时出现轻微浑浊，请在使用前通过离心澄清溶液。如果在12小时内未使用，则应丢弃工作稀释样品。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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存储类别

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

常规特殊物品

低风险生物材料

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Expression of one important chaperone protein, heat shock protein 27, in neurodegenerative diseases.

Xuekai Zhang et al.

Alzheimer's research & therapy, 6(9), 78-78 (2015-01-27)

Many neurodegenerative diseases are characterised by accumulations of misfolded proteins that can colocalise with chaperone proteins (for example, heat shock protein 27 (HSP27)), which might act as modulators of protein aggregation. The role of HSP27 in the pathogenesis of neurodegenerative

SQSTM1/p62 Promotes Cell Growth and Triggers Autophagy in Papillary Thyroid Cancer by Regulating the AKT/AMPK/mTOR Signaling Pathway.

Fangqin Yu et al.

Frontiers in oncology, 11, 638701-638701 (2021-05-04)

Thyroid cancer is one of the most common endocrine malignancies worldwide, and papillary thyroid cancer (PTC) is the most common pathologic type of thyroid cancer. SQSTM1/p62 activity mediates different biological functions. This study aimed to investigate the effect of SQSTM1/p62

Lysosomes, autophagosomes and Alzheimer pathology in dementia with Lewy body disease.

Rowan Gurney et al.

Neuropathology : official journal of the Japanese Society of Neuropathology (2018-05-12)

A failure of protein degradation may underpin Lewy body disease (LBD) where α-synuclein is assimilated into the pathognomic Lewy bodies and Lewy neurites. We investigated histological alterations in lysosomes and autophagosomes in the substantia nigra (SN) and cingulate gyrus (CG)

Expression of one important chaperone protein, heat shock protein 27, in neurodegenerative diseases

Zhang X, et al.

Alzheimer's Research & Therapy, 6(9), 78-78 (2014)

An activation of LC3A-mediated autophagy contributes to de novo and acquired resistance to EGFR tyrosine kinase inhibitors in lung adenocarcinoma.

Kaito Nihira et al.

The Journal of pathology, 234(2), 277-288 (2014-04-02)

The development of therapeutic resistance to EGFR tyrosine kinase inhibitors (EGFR-TKIs, ie erlotinib or gefitinib) has been the major clinical problem when treating lung adenocarcinoma patients with these agents. However, its mechanisms have not necessarily been well studied to this

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Anti-MAP1LC3A antibody

rabbit polyclonal

选择尺寸

关于此项目

主要文件

属性

产品名称

biological source

conjugate

antibody form

antibody product type

clone

form

mol wt

species reactivity

concentration

technique(s)

UniProt accession no.

shipped in

storage temp.

target post-translational modification

Quality Level

Gene Information

相关类别

说明

General description

Immunogen

Application

Biochem/physiol Actions

Physical form

Preparation Note

Disclaimer

安全信息

存储类别

wgk

flash_point_f

flash_point_c

ppe

法规信息

文件

分析证书(COA)

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