产品名称
来自 菜豆的凝集素 (红芸豆), Phytohemagglutinin PHA-M, lyophilized powder, BioReagent, suitable for cell culture
product line
BioReagent
form
lyophilized powder
potency
<40 μg per mL agglutination activity
technique(s)
cell culture | mammalian: suitable
impurities
salt, free
solubility
PBS: 2 mg/mL
storage temp.
2-8°C
Quality Level
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Analysis Note
凝集活性以μg/mL表示,并由1mg/ml溶液的连续稀释液进行测定。该活性是在25℃温育1小时后,在pH6.8的磷酸盐缓冲盐水中,凝集2%人红细胞悬浮液所需的最低浓度。
Application
来自菜豆(Phaseolus vulgaris) (红芸豆)的凝集素可用于淋巴细胞转化试验和培养基。
Biochem/physiol Actions
PHA由两种分子种类组成,即具有低促有丝分裂活性和高红细胞凝集素活性的红细胞凝集素(PHA-E),和具有高促有丝分裂和白细胞凝集活性但具有非常低的红细胞凝集活性的白细胞凝集素(PHA-L)。
PHA-E不具有血型特异性,但其凝集活性可被某些低聚糖抑制。 在分离和纯化红细胞凝集素和白细胞凝集素之前,PHA-P是PHA的蛋白质形式。 PHA-M是粘蛋白形式。 结合物由相应的纯化凝集素进行制备。
PHA-E不具有血型特异性,但其凝集活性可被某些低聚糖抑制。 在分离和纯化红细胞凝集素和白细胞凝集素之前,PHA-P是PHA的蛋白质形式。 PHA-M是粘蛋白形式。 结合物由相应的纯化凝集素进行制备。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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