Merck
CN

L9043

Sigma-Aldrich

溶葡球菌酶 来源于模仿葡萄球菌

recombinant, expressed in E. coli, lyophilized powder

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别名:
甘氨酰-甘氨酸内肽酶
CAS号:
MDL编号:
NACRES:
NA.54

生物来源

bacterial (Streptomyces globisporus)

质量水平

重组

expressed in E. coli

检测方案

≥90% (HPLC)

形式

lyophilized powder

比活

≥3000 unit/mg solid

技术

molecular cloning: suitable

适用性

suitable for sequence analysis test

应用

life science and biopharma

运输

dry ice

储存温度

−20°C

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一般描述

溶葡萄球菌酶是一种锌金属酶,主要以酶原的形式分泌出来。它可用作抗金黄色葡萄球菌剂,在pH4和5℃时高度稳定。

应用

来自拟葡萄球菌的溶葡球菌酶被用于评估lytM(一种独特的金黄色葡萄球菌自溶基因)的分子克隆、测序和表达。 它还被用于研究金黄色葡萄球菌中编码肽聚糖水解酶活性的基因的序列分析。

生化/生理作用

一种蛋白复合体,对葡萄球菌属(包括金黄色葡萄球菌)具有高度特异性裂解活性。
溶葡萄球菌酶具有抗金黄色葡萄球菌的裂解作用,可用于治疗耐抗生素的葡萄球菌感染。它具有甘氨酰甘氨酸内肽酶、内-β-N-乙酰葡萄糖胺酶和N-乙酰胞壁酰-L-丙氨酸酰胺酶等多种酶的特性。溶葡萄球菌酶还可用于食品防腐和临床实验室的快速筛查。
通过与金黄色葡萄球菌的膜相关Trap蛋白的C′ 端结合,溶葡球菌酶(金黄色葡萄球菌表达的一种金属内肽酶)的抗微生物活性得到增强。

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

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  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How should a stock solution of the Lysostaphin from Staphylococcus simulans enzyme, product L9043, be prepared?

    A stock solution of this recombinant, Lysostaphin from Staphylococcus simulans, may be prepared by reconstituting it at 10 mg/ml in 20 mM sodium acetate buffer, pH 4.5 and storing it aseptically at 4°C. in a polypropylene container. 

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C-terminus of TRAP in Staphylococcus can enhance the activity of lysozyme and lysostaphin.
Yang, G.
Acta Biochimica et Biophysica Hungarica, 40, 452-458 (2008)
L Ramadurai et al.
Journal of bacteriology, 179(11), 3625-3631 (1997-06-01)
A gene encoding an autolytic activity was identified in an autolysis-deficient mutant (Lyt-) of Staphylococcus aureus which produces only a single band in autolytic-activity gels (N. Mani, P. Tobin, and R. K. Jayaswal, J. Bacteriol. 175:1493-1499, 1993). An open reading
X Wang et al.
Gene, 102(1), 105-109 (1991-06-15)
The nucleotide (nt) sequence of a 2.0-kb NheI-XbaI DNA fragment containing a peptidoglycan hydrolase-encoding gene, lytA, tentatively identified as encoding an N-acetylmuramyl-L-alanine amidase, from Staphylococcus aureus, was determined. The nt sequencing revealed an open reading frame (ORF) of 1443 bp
Grégory Francius et al.
Journal of bacteriology, 190(24), 7904-7909 (2008-10-07)
The advent of Staphylococcus aureus strains that are resistant to virtually all antibiotics has increased the need for new antistaphylococcal agents. An example of such a potential therapeutic is lysostaphin, an enzyme that specifically cleaves the S. aureus peptidoglycan, thereby
Lysostaphin: an antistaphylococcal agent
Kumar J K
Applied Microbiology and Biotechnology, 80(4), 555-561 (2008)

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