General description
凝集素是植物来源的蛋白质，具有与某些神经元表面糖蛋白和糖脂结合的能力。它们的这种能力帮助它们被用作神经解剖示踪剂，因为它们以逆行或顺行的方式摄取和运输。
Application
来自 兵豆的凝集素用于使用 ANA 进行基于大小或电荷的蛋白质分离。（各向异性纳米过滤器阵列：ANA）。
Biochem/physiol Actions
LcH 不具有血型特异性。对末端 α-D-甘露糖基和 α-D-葡萄糖基残基有亲和力。LcH 由两种异构体组成，LcH-A（分子量 49,000）和 LcH-B（分子量 49,000）。LcH-A 具有促有丝分裂活性。结合物由纯化的凝集素制备。
Preparation Note
通过亲和层析高度纯化。
Analysis Note
凝集活性以 μ 表示g/mL，通过在 pH 6.8 磷酸盐缓冲盐水中连续稀释 1 mg/mL 溶液测定。该活性是在 25°C 下孵育 1 h 后凝集 2% 人红细胞悬液的最低浓度。
含有电泳上相应的于同工凝集素 LcH-A 和 LcH-B 的两条主带。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
低风险生物材料
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