sterility
aseptically filled
form
lyophilized powder
potency
≤0.3 μg/mL mitogenic activity
storage temp.
2-8°C
Quality Level
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General description
凝集素是一类普遍存在于真菌、植物和动物体内的碳水化合物结合蛋白。植物和动物中对凝集素的结构研究较多。该蛋白的二级结构富含 β-链，并在表面拥有一个碳水化合物结合部位。
Application
来自 美洲商陆的凝集素已被用于：
- 作为单核细胞培养的培养基补充物，以诱导淋巴细胞增殖
- 作为阳性对照研究趋化因子（C-C基序）配体2（CCL2）在蜕膜白细胞增殖和凋亡中的作用
- 作为RPMI（洛斯维·帕克纪念研究所）1640号培养基的补充剂，用于外周血淋巴细胞培养
Biochem/physiol Actions
PWM 从商陆根中提取。该蛋白质具有红血球凝集、白血球凝集和促有丝分裂特性。PWM 不具有血型特异性，但具有与 N-乙酰基-β-D-葡糖胺低聚物的亲和性。
凝集素在糖缀合物的表征、成像和靶向性方面具有重要作用。它在微阵列分析中的应用，使有效的糖组分析成为可能。这是因为其与寡糖、糖蛋白和糖脂的特异性相互作用。在植物和真菌中，凝集素防御病原体/食料。凝集素参与宿主的识别和组织的黏附，参与微生物的致病过程。
Physical form
部分纯化的 TCA 沉淀。
基本不含盐。
基本不含盐。
Analysis Note
通过将 BrdU 掺入 HeLa 细胞培养基中测定有丝分裂促进活性。
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
低风险生物材料
此项目有
The use of a novel combination of diagnostic molecular and cytogenetic approaches in horses with sexual karyotype abnormalities: a rare case with an abnormal cellular chimerism
Demyda P, et al.
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Assessment of cellular immune responses of healthy and diseased Tasmanian devils (Sarcophilus harrisii)
Kreiss A, et al.
Developmental and Comparative Immunology, 32(5), 544-553 (2008)
B Hejdeman et al.
HIV medicine, 4(2), 101-110 (2003-04-19)
To study the impact of effective highly active antiretroviral therapy (HAART) on the preservation of long-term CD4 memory cells induced by vaccines in HIV-1-infected patients. Thirty HIV-1-positive patients on HAART with undetectable viral load were randomized into three groups: 10
The immune response of the Tasmanian devil (Sarcophilus harrisii) and devil facial tumour disease
Woods GM, et al.
EcoHealth, 4(3), 338-345 (2007)
W E Müller et al.
European journal of biochemistry, 183(2), 391-396 (1989-08-01)
Previously it was established [Pahwa, S., Pahwa, R., Saxinger, C., Gallo, R. C. & Good, R. A. (1985) Proc. Natl. Acad. Sci. USA 82, 8198] that nonviable preparations of human immunodeficiency virus 1 (HIV-1) abolish the proliferative response of human
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