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Merck
CN

L9420

荧光素酶 来源于北美萤火虫 (萤火虫)

recombinant, expressed in E. coli, buffered aqueous solution, ≥10×1010 units/mg protein

别名:

荧光素酶

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关于此项目

化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥10×1010 units/mg protein
Assay:
≥98% (SDS-PAGE)
Recombinant:
expressed in E. coli
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产品名称

荧光素酶 来源于北美萤火虫 (萤火虫), recombinant, expressed in E. coli, buffered aqueous solution, ≥10×1010 units/mg protein

recombinant

expressed in E. coli

assay

≥98% (SDS-PAGE)

form

buffered aqueous solution

specific activity

≥10×1010 units/mg protein

mol wt

62 kDa

shipped in

dry ice

storage temp.

−20°C

Quality Level

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Application

来自 Photinus pyralis (萤火虫)的荧光素酶已被用作裂解溶液的组分,以测量发光信号,作为确定可激活 karrikin 不敏感 2 (KAI2) 途径的化学信号的研究的一部分。

General description

萤火虫荧光素酶催化荧光素与 ATP 的反应,并导致黄绿色光的产生。该酶分子量为 62 kDa,在过氧化物酶体中表达。荧光素酶被认为是研究蛋白质-麻醉剂相互作用的模型。萤火虫荧光素酶在细胞生物学和分子生物学中非常有用,可作为基因功能的报告基因以及用于 ATP 的定量。

Other Notes

一个荧光素酶单位在 20-25℃ 下将在10 秒内产生一个相对光单位 (RLU),使用 GloMax 20/20 光度计在Tris-甘氨酸缓冲液中含有40pmol ATP和15nmol荧光素的100μl测定混合物中测定,溶液PH值为7.6。

单位定义换算系数:每个传统照明单位大约有 9000 个相对照明单位 (RLU),在PPO / POPOP混合物中使用相当于0.02μ Ci 14 C 的峰高。

Physical form

提供溶于 25 mM Tris-乙酸盐(pH 7.8)、1 mM EDTA、1 mM DTT、0.2M 硫酸铵、15% 甘油和 30% 乙二醇的 a 溶液

pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Resp. Sens. 1 - STOT RE 2 Oral

target_organs

Kidney

存储类别

10 - Combustible liquids

wgk

WGK 3

flash_point_f

231.8 °F

flash_point_c

111 °C

法规信息

常规特殊物品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes.
Conti E, et al.
Structure, 4(3), 287-298 (1996)
Griffiths A J F, et al.
An Introduction to Genetic Analysis. (2000)
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Cell reports, 42(3), 112167-112167 (2023-03-02)
mRNA vaccines are effective in preventing severe COVID-19, but breakthrough infections, emerging variants, and waning immunity warrant the use of boosters. Although mRNA boosters are being implemented, the extent to which pre-existing immunity influences the efficacy of boosters remains unclear.
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Artificial cells (ACs) aim to mimic selected structural and functional features of mammalian cells. In this context, energy generation is an important challenge to be addressed when self-sustained systems are desired. Here, mitochondria isolated from HepG2 cells are employed as

商品

Bioluminescence imaging (BLI) systems allows for high-sensitive and noninvasive monitoring of cell proliferation, activity of signaling pathways and protein-protein interactions in living tissues.

Firefly luciferase is a sensitive reporter for gene studies due to its absence in mammalian cells or tissues.

seMpai substrate is suitable for near-infrared BLI in biological experiments, offering high solubility for extended bioluminescent applications.

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