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Merck
CN

L9504

D -荧光素

luminescent, ≥98% (HPLC), powder

别名:

( S )-2-（6-羟基-2-苯并噻唑基）-2-噻唑啉-4-羧酸, 4,5-二氢-2-（6-羟基-2-苯并噻唑基）-4-噻唑羧酸, 萤火虫荧光素

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关于此项目

经验公式（希尔记法）:
C11H8N2O3S2
化学文摘社编号:
2591-17-5
分子量:
280.32
NACRES:
NA.32
PubChem Substance ID:
24896524
UNSPSC Code:
12352204
EC Number:
219-981-3
MDL number:
MFCD00042929
Beilstein/REAXYS Number:
30484

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产品名称

D -荧光素, synthetic

InChI key

BJGNCJDXODQBOB-SSDOTTSWSA-N

InChI

1S/C11H8N2O3S2/c14-5-1-2-6-8(3-5)18-10(12-6)9-13-7(4-17-9)11(15)16/h1-3,7,14H,4H2,(H,15,16)/t7-/m1/s1

SMILES string

OC(=O)[C@H]1CSC(=N1)c2nc3ccc(O)cc3s2

biological source

synthetic

assay

≥98% (HPLC)

form

powder

solubility

DMSO: 10 mg/mL, clear, colorless to faintly yellow

fluorescence

λex 328 nm; λem 532 nm in H2O

storage temp.

−20°C

Quality Level

300

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Application

Km 约为 2 μM 的萤火虫荧光素酶的底物。
荧光素用于通过荧光素酶检测测定小鼠和拟南芥种子中的荧光素酶活性。它也被用于测量新生大鼠心室心肌细胞的生物发光。

Biochem/physiol Actions

细胞内的荧光素摄入受到三磷酸腺苷(ATP)结合盒(ABC)转运体抑制剂的调节，例如ATP结合盒转运子G2 (ABCG2)/乳腺癌耐受蛋白(BCRP)抑制剂烟曲霉毒素C等。这一基质被萤火虫萤光素酶氧化，发出能够穿过活组织的光量子。

General description

荧光素是一种大量存在于发光性生物体中的小分子。该分子对氧和光敏感。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000455378
0000455377
0000455355
0000455377
0000454231

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