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Merck
CN

L9514

蓖麻毒素A链 来源于蓖麻 (蓖麻子)

buffered aqueous glycerol solution

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关于此项目

化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.32
MDL number:
Form:
buffered aqueous glycerol solution
Storage temp.:
2-8°C
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form

buffered aqueous glycerol solution

Quality Segment

potency

≥250 μg/mL per mL agglutination activity

composition

Protein, ≥0.3 mg/mL Lowry-TCA

storage temp.

2-8°C

Biochem/physiol Actions

Ricin A chain inactivates the 60 S ribosomal subunit of eukaryotic cells, inhibiting protein synthesis. This toxic activity is independent of the presence of the B chain of ricin.

Physical form

Solution in 40% glycerol containing 10 mM phosphate, pH 6.0, 0.15 M NaCl, 10 mM galactose and 0.5 mM dithioerythritol

Preparation Note

Purified by affinity chromatography.

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 7.2, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hour incubation at 25°C.
Electrophoretically pure (SDS-PAGE)

Other Notes

Ricinus communis toxin (also named RCA60, RCAII, Ricin D, or RCL III) is a highly toxic protein, mol. wt. ~60 kDa, composed of two chains, A and B, connected by a single disulfide bridge.


pictograms

Skull and crossbones

signalword

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Skin Sens. 1

存储类别

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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