Merck
CN

L9655

Sigma-Aldrich

亚油酸-油酸-白蛋白,100x

liquid, sterile-filtered, BioReagent, suitable for cell culture

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别名:
Fatty Acid-Albumin-complexes
NACRES:
NA.75

生物来源

protein from bovine

无菌性

sterile-filtered

产品线

BioReagent

形式

liquid

组成

linoleic acid and oleic acid, 2 mol/mol albumin (each)

浓度

100 mg/mL BSA in DPBS

technique(s)

cell culture | mammalian: suitable

杂质

endotoxin, tested

储存温度

2-8°C

一般描述

脂肪酸-白蛋白复合物在无血清培养基的研制中用作脂质的替代来源。其中脂肪酸以高配比与血清蛋白质相结合。这些蛋白质可以释放有益脂肪酸并结合那些有抑制作用的脂肪酸。油酸与牛血清白蛋白(BSA)结合有利于多种细胞类型(例如幼仓鼠肾 BHK)的生长。多不饱和和单不饱和脂肪酸(如,亚油酸和油酸)在用作培养基补充剂使用时会产生协同效应。

应用

亚油酸-油酸-白蛋白(100x)用于:
  • 诱导原代肝细胞中的急性肝细胞应激
  • 诱导滋养层细胞中的氧化应激
  • 诱导HepG2 细胞中的脂滴积聚

相关产品

产品编号
说明
价格

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves

法规信息

含少量动物源组分生物产品

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Pierluigi Ramadori et al.
Free radical biology & medicine, 91, 114-126 (2015-12-25)
Generation of reactive oxygen species (ROS) in response to fatty acids accumulation has been classically proposed as a possible "second hit" triggering progression from simple steatosis to non-alcoholic steatohepatitis (NASH). In this study we challenged hepatocyte-specific Keap1 knockout mice (Keap1(Δhepa))
Laura Moody et al.
Biochimica et biophysica acta. Gene regulatory mechanisms, 1862(2), 141-152 (2019-01-04)
Carnitine palmitoyltransferase 1 (Cpt1a) is a rate-limiting enzyme that mediates the transport of fatty acids into the mitochondria for subsequent beta-oxidation. The objective of this study was to uncover how diet mediates the transcriptional regulation of Cpt1a. Pregnant Sprague Dawley
Liang Tian et al.
International journal of molecular sciences, 20(5) (2019-03-13)
This study aimed to characterize the full-length cDNA of MARK4 in Sus scrofa, and evaluated its potential role in the regulation of lipid accumulation in pig placental trophoblasts and analyzed signaling pathways involved, thereby providing insights into mechanisms for placental
Yukimasa Takeda et al.
Scientific reports, 10(1), 3775-3775 (2020-03-01)
Brown adipocytes coordinate systemic energy metabolism associated with the pathogenesis of obesity and related metabolic diseases including type 2 diabetes. We have previously reported chemical compound-induced brown adipocytes (ciBAs) converted from human dermal fibroblasts without using transgenes. In this study
Jan Frohlich et al.
Biochimica et biophysica acta. Molecular and cell biology of lipids, 1866(6), 158920-158920 (2021-03-09)
Hepatocellular carcinoma (HCC) is one of the fastest-growing causes of cancer-related mortalities worldwide and this trend is mimicked by the surge of non-alcoholic fatty liver disease (NAFLD). Altered hepatic lipid metabolism promotes HCC development through inflammation and activation of oncogenes.

商品

Importance and uses of linoleic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

How the unsaturated fatty acid, oleic acid and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.

不饱和脂肪酸、油酸和其他细胞培养成分如何影响无血清、无蛋白细胞培养系统(用于生物制造包括单克隆抗体在内的异源蛋白)的性能。

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