Name: Lentiviral Pools
Brand: SIGMA

CRISPR Lentrivirus<br>Order - GeCKOv2 Pool

<h2>Create Your Own <span class="h2-lowercase">sh</span>RNA Pool</h2>


<h2>Knockout Genes using our CRISPR Pooled Lentivirus</h2>
<h3>CRISPR Whole Genome Pools</h3>


<h2>Knockdown Genes using our <span class="h2-lowercase">sh</span>RNA Pooled Lentivirus</h2>
<h3>shRNA Whole Genome Pools</h3>


<h2><span class="h2-lowercase">sh</span>RNA Human Kinase Pools</h2>


<h2>Overexpress Genes using our L<span class="h2-lowercase">enti</span>ORF Pooled Lentivirus</h2>
<h3>Pooled Human LentiORF Libraries</h3>


<h3>Whole genome CRISPR knockout screening for every lab</h3>
<ul>
<li>Utilize the power of CRISPR to knockout and screen every gene in the human genome</li>
<li>Select from the GeCKOv2 or our Whole Human Genome CRISPR Pools</li>
<li>Average of 6 gRNAs per gene (GeCKOv2); 10 gRNAs per gene</li>
<li>Divided into 2 subpools at 62,000 clones per pool (GeCKOv2, 124,000 clones total);8 subpools at 23,000 clones per pool (184,000 clones total)</li>
<li>Numerous built-in enrichment, depletion, and non-targeting control clones allow researchers to confidently gauge the success of their pooled screening experiments</li>
<li>2 vector system (pools are gRNA-only, Cas9 sold separately)</li>
<li>All pools are deep sequenced to verify clone representation and distribution</li>
<li>Deliverables: 200 μl of each pool at a minimum of 5x108 viral particles/ml (via p24), ships immediately</li>
</ul>


<h4>Create Your Own CRISPR Pool - Focus on the genes essential to your research</h4>
<ul>
<li>Special promotion: 10,000 pre-designed clones for $12,000</li>
<li>Custom viral titers, volumes, and aliquoting are available (standard deliverable: 200 μl at a minimum of 5 x 10<sup>8</sup> viral particles/ml in 4 x 50 μl aliquots); quotes available upon request</li>
<li>All pools are deep sequenced to verify clone representation and distribution</li>
<li>TAT: 8-10 weeks from finalization of clone list</li>
<li>DNA format also available</li>
</ul>


<h4>Focus on the genes essential to your research by creating your own custom shRNA pool</h4>
<ul>
<li>Create a custom shRNA pool to specifically target your genes of interest</li>
<li>Customize your viral titers, volumes, and aliquoting needs for greater flexibility in your screening experiments. DNA format also available.</li>
<li>Titers and volumes adequate for <i>in vitro</i>, <i>in vivo</i> and xenograft applications</li>
<li>Partner with our next-generation sequencing services for data deconvolution to get the most out of your assays</li>
</ul>


<h4>Whole genome overexpression ORF screening at a fraction of the cost</h4>
<ul>
<li>Exclusive to us - Puromycin expressing LentiORF library barcoded for pooled screening applications</li>
<li>Each ORF Clone contains a unique 24 NT barcode sequence for easy deconvolution and identification of hits</li>
<li>Perform overexpression screening to study cancer phenotypes</li>
<li>Trusted TRC3 (The RNAi Consortium) content with ORF gene expression spanning 14,000+ human genes and 17,000+ clones</li>
<li>Simplified whole genome ORF screening in standard or difficult to expand cell lines</li>
</ul>


<h4>Survey the entire human kinome for novel hits with pooled CRISPR knockout clones</h4>
<ul>
<li>Over 6,000 unique lentiviral clones expressing both Cas9 and gRNA sequences designed to functionally inactivate (knockout) nearly 700 human kinase genes</li>
<li>High clone coverage (7-10 distinct gRNAs per kinase gene, average of 8.6 per gene) increases the statistical strength of your hit analysis</li>
<li>Numerous built-in enrichment and depletion controls</li>
<li>The pool contains 100 non-targeting control gRNAs as normalization standards</li>
<li>The human kinase pool is deep sequenced to verify clone representation and distribution</li>
<li>gRNA design and tiling was created with a stringent bioinformatics approach to minimize the potential for off-targeting</li>
</ul>


<h4>Knockdown the genome</h4>
<ul>
<li>Trusted TRC (The RNAi Consortium) content with shRNAs targeting 20,000+ human or 21,000+ mouse genes including TRC 1.5 and 2.0 (unique and validated) add-ons which are exclusive to us</li>
<li>Enjoy the power of lentiviral transduction for screening in standard or difficult to expand cell lines.</li>
<li>Deliverables: 50 μl of each pool at a minimum of 5x10<sup>8</sup> viral particles/ml (via p24), ships immediately</li>
<li>Versatile and compatible with FACS and most downstream fluorescent assays</li>
<li>Affordable and easy-to-use allowing for bench-top discovery without robotics or liquid handling</li>
</ul>


<h4>Knockdown and focus on the kinase gene pathway</h4>
<ul>
<li>Over 9,400 unique lentiviral shRNA clones to knockdown 740+ human kinase genes</li>
<li>High clone coverage (average of 12.6 clones per gene) increases the statistical strength of your hit analysis</li>
<li>Deliverables: 100 μl of each pool at a minimum of 5x10<sup>8</sup> viral particles/ml (via p24), ships immediately</li>
<li>Numerous built-in enrichment, depletion, and non-target control clones allows researchers to confidently gauge the success of their pooled screening experiments</li>
</ul>


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Lentiviral Pools Knockdown (RNAi), Knockout (CRISPR), and Express (LentiORF); Synonyms: CRISPR knockdown,Custom CRISPR Pooled Library,Custom ORF Lentiviral Overexpression Pool,Custom shRNA Pooled Library,Gecko2 Human Whole Genome CRISPR Pool,Gecko2 Mouse Whole Genome CRISPR Pool,Human Kinase Lentiviral CRISPR Pool,MISSION® Human Kinase shRNA Lentiviral Pool,MISSION® LentiPlex® Human Pooled shRNA Library,MISSION® LentiPlex®Mouse Pooled shRNA Library,MISSION® TRC3 LentiORF Whole Genome Lentiviral Pool,Whole Human Genome Lentiviral CRISPR Pool at Sigma-Aldrich

Lentiviral Pools Knockdown (RNAi), Knockout (CRISPR), and Express (LentiORF) | Sigma-Aldrich

Knockdown (RNAi), Knockout (CRISPR), and Express (LentiORF)

Lentiviral Pools Knockdown (RNAi), Knockout (CRISPR), and Express (LentiORF)

Lentiviral Pools

Synonym: CRISPR knockdown, Custom CRISPR Pooled Library, Custom ORF Lentiviral Overexpression Pool, Custom shRNA Pooled Library, Gecko2 Human Whole Genome CRISPR Pool, Gecko2 Mouse Whole Genome CRISPR Pool, Human Kinase Lentiviral CRISPR Pool, MISSION® Human Kinase shRNA Lentiviral Pool, MISSION® LentiPlex® Human Pooled shRNA Library, MISSION® LentiPlex®Mouse Pooled shRNA Library, MISSION® TRC3 LentiORF Whole Genome Lentiviral Pool, Whole Human Genome Lentiviral CRISPR Pool

主要文件

Lentiviral Pools

Knockdown (RNAi), Knockout (CRISPR), and Express (LentiORF)

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