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Merck
CN

LGC600511

E.Cloni 10G Elite (SOLOs) Electrocompetent Cells

Cfu/μg >2 x 1010, pkg of 12 reactions (12 x 25 µL)

别名:

DNA cloning, Electrocompetent E.Coli, Electrocompetent cells, Electroporation, Standard cloning competent cells, competent cells

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UNSPSC代码:
41106202

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生物来源

Escherichia coli

制造商/商品名称

Lucigen 60051-1

包装

pkg of 12 reactions (12 x 25 µL)

生长模式

growth or suspension

技术

microbiological culture: suitable

细胞转化

competent cell type: electrocompetent
transformation efficiency: 2 x 1010 cfu/μg

运输

dry ice

储存温度

-65 to -96°C

相关类别

一般描述

E. cloni electrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability. These cells provide solutions for a wide range of applications at economical prices.

Packaged as SOLOs (1 transformation per tube, 12 x 25µl)

应用

E. cloni 10G Competent Cells. Library construction, cloning, subcloning, and plasmid isolation with or without blue/white screening.

特点和优势

  • Optimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable (without IPTG induction)
  • Supports cloning methylated DNA
  • Replace standard cloning strains (e.g., OP10, DH5α, DH10B, XL1-Blue, etc.)

Transformation efficiency: ≥ 2 x 1010 (cfu/µg pUC DNA)

Genotype: E.cloni 10G: F¯ mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 ɸ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL (StrR) nupG λ- tonA

Components


  • E. cloni 10G Elite Electrocompetent Cells
  • pUC 19 Transformation Control
  • Recovery Media

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