biological source
Escherichia coli
manufacturer/tradename
Lucigen 60052-4
packaging
pkg of 48 reactions (8 x 150 µL)
growth mode
growth or suspension
technique(s)
microbiological culture: suitable
cell transformation
competent cell type: electrocompetent
transformation efficiency: 2 x 1010 cfu/μg
shipped in
dry ice
storage temp.
-65 to -96°C
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General description
E. cloni electrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability. These cells provide solutions for a wide range of applications at economical prices.
Application
E. cloni 10G Competent Cells. Library construction, cloning, subcloning, and plasmid isolation with or without blue/white screening.
Features and Benefits
- Optimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable (without IPTG induction)
- Supports cloning methylated DNA
- Replace standard cloning strains (e.g., OP10, DH5α, DH10B, XL1-Blue, etc.)
Transformation efficiency: ≥ 2 x 1010 (cfu/µg pUC DNA)
Genotype: E. cloni 10G: F¯ mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 ɸ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL (StrR) nupG λ- tonA
Components:
E. cloni 10G Elite Electrocompetent Cells
pUC 19 Transformation Control
Recovery Media
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