生物来源
Escherichia coli
制造商/商品名称
Lucigen 60052-4
包装
pkg of 48 reactions (8 x 150 µL)
生长模式
growth or suspension
技术
microbiological culture: suitable
细胞转化
competent cell type: electrocompetent
transformation efficiency: 2 x 1010 cfu/μg
运输
dry ice
储存温度
-65 to -96°C
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一般描述
E. cloni electrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability. These cells provide solutions for a wide range of applications at economical prices.
应用
E. cloni 10G Competent Cells. Library construction, cloning, subcloning, and plasmid isolation with or without blue/white screening.
特点和优势
- Optimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable (without IPTG induction)
- Supports cloning methylated DNA
- Replace standard cloning strains (e.g., OP10, DH5α, DH10B, XL1-Blue, etc.)
Transformation efficiency: ≥ 2 x 1010 (cfu/µg pUC DNA)
Genotype: E. cloni 10G: F¯ mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 ɸ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL (StrR) nupG λ- tonA
Components:
E. cloni 10G Elite Electrocompetent Cells
pUC 19 Transformation Control
Recovery Media
法规信息
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