biological source
Escherichia coli
manufacturer/tradename
Lucigen 60117-2
packaging
pkg of 48 reactions (8 x 150 µL)
growth mode
growth or suspension
technique(s)
microbiological culture: suitable
cell transformation
competent cell type: chemically competent
transformation efficiency: 1 x 107 cfu/μg
shipped in
dry ice
storage temp.
-65 to -96°C
General description
E. cloni 10G CLASSIC Electrocompetent Cells are high efficiency cells with a substantially lower cost per reaction. These cells are the most economical choice for standard cloning and library construction. 10G CLASSIC Cells are available in larger package sizes for convenient use in higher volume cloning applications.
Application
Clone, construct libraries, transform Gibson Assembly reactions all with high-yield, T1 phage-resistant cells in high efficiencies for routine and challenging applications
Features and Benefits
- Optimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable (without IPTG induction)
- Replace standard cloning strains (e.g., OP10, DH5α, DH10B, XL1-Blue, etc.)
- Supports cloning of methylated DNA
Transformation efficiency: ≥ 5 x 109 (cfu/µg pUC DNA)
Genotype: E. cloni 10G: F - mcrA ∆(mrr-hsdRMS-mcrBC) endA1 recA1 φ80dlacZ∆M15 ∆lacX74 araD139 ∆(ara,leu)7697 galU galK rpsL (StrR) nupG λ− tonA
Components:
E. cloni 10G Classic SixPacks Electrocompetent Cells
pUC 19 Transformation Control
Recovery Media
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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