biological source
Escherichia coli
manufacturer/tradename
Lucigen 60240-2
packaging
pkg of 24 reactions (12 x 80 µL)
growth mode
growth or suspension
technique(s)
microbiological culture: suitable
cell transformation
competent cell type: chemically competent
transformation efficiency: 1×107 cfu/μg
shipped in
dry ice
storage temp.
-65 to -96°C
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General description
Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be propagated stably.
Application
- Cloning and maintaining unstable or repetitive DNA sequences.
- Recommended for high efficiency propagation of GeCKO CRISPR libraries (1)
- Ideal for single-guide RNA lentiviral library construction (2)
Features and Benefits
- Clone repetitive sequences and lentiviral libraries
- Stabilize direct repeats and create lentiviral constructs
Transformation efficiency: ≥ 1 x 107 (cfu/µg)
Genotype: Endura: recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ– leu mtl-1 F– mcrB mrr hsdS20(rB–, mB–)
Components:
Endura Chemically Competent Cells
pUC 19 Trasformation Control
Recovery Media
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