生物来源
Escherichia coli
制造商/商品名称
Lucigen 60401-1
包装
pkg of 12 reactions (6 x 80 µL)
生长模式
growth or suspension
技术
microbiological culture: suitable
细胞转化
competent cell type: chemically competent
transformation efficiency: 1×107 cfu/μg
运输
dry ice
储存温度
-65 to -96°C
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一般描述
E. cloni EXPRESS BL21(DE3) Chemically Competent Cells are ideal for routine protein expression applications. These strain carries a chromosomal copy of the T7 RNA Polymerase gene under the control of the lacUV5 promoter and it is suitable for production of protein from target genes cloned into T7 driven expression vectors.
应用
Cloning, routine protein expression
特点和优势
- Routine protein expression
- High transformation efficiency
- Short protein expression protocol time
Transformation efficiency: ≥ 1 x 107 (cfu/µg pUC19)
Genotype: F – ompT hsdSB (rB- mB-) gal dcm (DE3)
Components:
E.Cloni EXPRESS BL21 (DE3) Electrocompetent Cells
pUC 19 Trasformation Control
Recovery Media
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