生物来源
Escherichia coli
制造商/商品名称
Lucigen 60502-2
包装
pkg of 24 reactions (12 x 50 µL)
生长模式
growth or suspension
技术
microbiological culture: suitable
细胞转化
competent cell type: electrocompetent
transformation efficiency: 4 x 1010 cfu/μg
运输
dry ice
储存温度
-65 to -96°C
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一般描述
TG1 Electrocompetent Cells deliver ≥4 x 1010 cfu/μg of DNA and are particularly useful for phage display protein expression. TG1 cells are also suitable for M13 phage work, general cloning, blue/white screening and protein expression.
应用
Phage Display Libraries
Protein expression
Protein expression
特点和优势
- Phage display libraries screening and protein expression
- Amber suppressor
- Supports cloning of methylated DNA
- White/blue screening-capable (IPTG induction)
Transformation efficiency: ≥ 4 x 1010 (cfu/µg pUC19)
Genotype: TG1: [F’ traD36 proAB lacIqZ ΔM15] supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5(rK - mK -)
Components:
TG1 Competent Cells
pUC 19 Trasformation Control
Recovery Media
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