TransforMax EC100D pir+ Electrocompetent E. coli

The TransforMax EC100D pir + Electrocompetent E. coli and TransforMax EC100D pir-116 Electrocompetent E. coli each constitutively express the π protein (the pir gene product) for replication of plasmids containing the R6Kγ origin of replication (R6Kγ ori). The cells are derived from Epicentre′s TransforMax EC100 Electrocompetent E. coli by P1 phage transduction with a strain containing the pir + or pir-116 gene linked to a dihydrofolate reductase (DHFR) marker.

The TransforMax EC100D pir + cells will maintain R6Kγ ori containing plasmids at approximately 15 copies per cell, 1 for cloning of potentially toxic or unstable DNA sequences. The TransforMax EC100D pir-116 cells are for high-copy propagation of up to 250 rescue plasmid copies per cell.

Packaged as 5 tubes x 100 µL of electrocompetent cells, which is enough cells for 10 electroporations

Plasmid rescue for transposition mutants created with the EZ-Tn5 Transposome Kits

• Rescue cloning of EZ-Tn5 transposed elements
• Propagate plasmids tagged with the EZ-Tn5 Insertion Kit
• Maintains plasmids at ~15 copies per cell.

Transformation efficiency: ≥ 5 x 109 (cfu/µg supercoiled DNA)

Genotype TransforMax EC100D pir+116 Electrocompetent E. coli: F - mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (Str R) nupG pir +(DHFR)

Components:
TransforMax EC100D pir+ Electrocompetent E. coli
pR6Kan Control Plasmid
Recovery Media