manufacturer/tradename
Lucigen QEF81050
packaging
pkg of 50 mL, pkg of 500 reactions
technique(s)
DNA extraction: suitable
General description
The analysis of nucleic acid from formalin-fixed, paraffin-embedded (FFPE) specimens is challenging due to the extensive cross-linking of all tissue components during the fixation process. These challenges include chemical modification of the DNA, cross-linking of DNA with other molecules, degradation of the DNA, and the limited amount of nucleic acid in the samples.
The QuickExtract FFPE DNA Extraction Kit is a fast, simple, and inexpensive method for preparing genomic DNA for PCR amplification from archival samples. The protocol requires only heat treatment to melt the paraffin, lyse the cells, decrease the fomalin-induced cross-linking in the sample, and degrade compounds inhibitory to amplification. Following heat treatment, the sample DNA is ready for PCR
The QuickExtract FFPE DNA Extraction Kit is a fast, simple, and inexpensive method for preparing genomic DNA for PCR amplification from archival samples. The protocol requires only heat treatment to melt the paraffin, lyse the cells, decrease the fomalin-induced cross-linking in the sample, and degrade compounds inhibitory to amplification. Following heat treatment, the sample DNA is ready for PCR
Application
Isolation of DNA from FFPE samples for PCR-based analysis, e.g., microsatellite detection, SNP detection, tumour heterogeneity studies, copy number detection, methylation analysis, and Short Tandem Repeat (STR) analysis
Features and Benefits
- Simple, rapid extraction of PCR-ready DNA from challenging FFPE samples
- Fast: PCR-ready DNA in minutes, not days
- Simple: No spin columns or transfer steps which helps increase yields
- Compatible: Extracted DNA is compatible with both real-time and end-point PCR
- Safe: No xylene or phenol extractions used
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1A - STOT SE 3
target_organs
Respiratory system
存储类别
8B - Non-combustible corrosive hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable