Terminator 5′-Phosphate-Dependent Exonuclease

Terminator^® 5´-Phosphate-Dependent Exonuclease* is a processive 5´ to ;3´ exonuclease that digests RNA that has a 5´-monophosphate end. It does not digest RNA that has a 5´-triphosphate, 5´-cap or 5´-hydroxyl group. Terminator Exonuclease is not inhibited by proteinaceous RNase inhibitors, such as RiboGuard RNase Inhibitor or placental ribonuclease inhibitors

• Specific: Processively digests RNA with 5´-monophosphate ends but not RNAs with 5´-triphosphate, 5´-cap or 5´-hydroxyl groups starting from the 5´ end
• Flexible: Use this enzyme to help characterise 5´-ends of RNA and to enrich for mRNA in prokaryotic or eukaryotic total RNA preparations

Unit Definition: One unit of Terminator^® Exonuclease digests 1 µg of rRNA substrate to acid-soluble nucleotides in 60 minutes at 30 °C under standard assay conditions.

Storage Buffer: 50% glycerol containing 0.05 M Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton^™ X-100.

Kit Components:
Terminator Exonuclease
Terminator 10X Reaction Buffer A
Terminator 10X Reaction Buffer B

DNATerminator is a registered trademark of Lucigen Corporation

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow