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Merck
CN

LH20100

Sephadex® LH-20 Size Exclusion Resin

140-550 mesh

别名:

亲脂交联葡聚糖®

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UNSPSC Code:
13111000
NACRES:
NB.21

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产品名称

Sephadex® LH-20,

form

slurry

technique(s)

LPLC: suitable

matrix

Agarose

matrix active group

polymer

swelling

1 g swells to 4 mL (water, methanol)

bead size

25-100 μm

operating pH range

2-13

separation technique

size exclusion (SEC)

storage temp.

room temp

Quality Level

100

相关类别

Application

Sephadex® Lh-20是一种交联葡聚糖基树脂，可结合有机溶剂流动相用于凝胶渗透、正相分配，以及脂质、类固醇、脂肪酸、激素，维生素等的吸附色谱层析。此外，这一产品还用于凝胶过滤色谱、蛋白质色谱，凝胶过滤介质、树脂和分离介质。
Sephadex® Lh-20已用于从黑莓基因型中分离生物活性多酚成分，以及从莲藕根茎水性提取物中分离色氨酸。

Legal Information

Sephadex is a registered trademark of Cytiva

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000528087
0000528087
0000524252
0000524252
0000479087

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is Sephadex LH-20, Product LH20100?

    Sephadex LH-20 is a beaded, cross-linked dextran which has been hydroxypropylated to yield a chromatographic media with both hydrophilic and lipophilic character.  Due to its dual character, LH-20 swell in water and a number of organic solvents.  The particle size and exclusion limit vary depending on the solvent used for swelling.  The swollen resin can be used for gel filtration separations of both hydrophilic and lipophilic compounds.

  4. What solvents can be used with Sephadex LH-20, Product LH20100?

    Sephadex LH-20 swells in water, polar organic solvents, aqueous solvent mixtures, and mixtures of polar organic solvents.  In solvent mixtures containing both polar and non-polar organic solvents the gel takes up predominantly the polar component.   Following is a table of solvents and the approximate swelling volume.  Solvents giving a bed volume of less than 2.5 ml/g dry gel are generally not used.Solvent                           Approx. bed volume (ml/g dry gel)Dimethyl sulfoxide           4.4 - 4.6Pyridine                          4.2 - 4.4Water                             4.0 - 4.4Dimethyl formamide        4.0 - 4.4Methanol                        3.9 - 4.3Chloroform                     3.8 - 4.1Ethanol                           3.6 - 3.9Methylene dichloride       3.6 - 3.9Tetrahydrofuran              3.3 - 3.6Acetone                          2.4 - 2.6Acetonitrile                     2.2 - 2.4Benzene                         1.6 - 2.0Ethyl acetate                   1.6 - 1.8Toluene                          1.5 - 1.6

  5. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  6. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Suman B Sharma et al.
Journal of medicinal food, 14(4), 353-359 (2011-03-05)
Diabetes is accompanied by lipid abnormalities, which contribute significantly to cardiovascular morbidity and mortality in diabetic patients. We previously demonstrated the potent antihyperglycemic activity of the active principle (fraction II from Sephadex LH 20 chromatography [LH II]) isolated from ethanolic
S K Kundu et al.
Journal of lipid research, 20(7), 825-833 (1979-09-01)
Aminopropyl silica gel was prepared from porous silica gel and was used as a solid support for immunoadsorbent in the purification of anti-glycolipid antibodies. For neutral glycosphingolipids, a carboxyl function was generated by oxidation of the olefinic double bond of
Yizhong Cai et al.
Journal of chromatographic science, 43(9), 454-460 (2005-10-11)
HPLC characterization of reversed-phase (RP) high-performance liquid chromatography (HPLC) has been widely used in separation and identification of plant pigments. An effective RP-HPLC-based method is established to systematically isolate, identify, and quantitate the betalain pigments in the plants of 37
D Pechinot et al.
Journal of steroid biochemistry, 18(5), 601-606 (1983-05-01)
A procedure for the determination of corticosterone in 20 microliters of rat plasma, in maternal and foetal late pregnancy with a protein binding radioassay, is described. The method is suitable in a range of 0.5-3 ng of corticosterone. An amount
N X Chen et al.
Journal of animal science, 73(3), 722-727 (1995-03-01)
Previous studies have shown that the responsiveness of porcine preadipocytes to glucocorticoids increases progressively with fetal age. In the current study, the development of fetal glucocorticoid receptors in porcine preadipocytes was examined in primary cultures derived from 50-, 75-, and
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实验方案

Column Packing & Separation with Sephadex® LH-20

Separation using Sephadex LH-20 column for Size Exclusion Chromatography media in polysaccharide network from Cytiva.

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