M042030
Biotin Phosphoramidite
configured for ABI
别名:
Biotin amidite
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关于此项目
经验公式（希尔记法）:
C46H64N5O8PS
化学文摘社编号:
分子量:
878.07
NACRES:
NA.21
PubChem Substance ID:
UNSPSC Code:
41116105
MDL number:
Assay:
≥96% (31P-NMR)
Storage temp.:
−20°C
InChI
1S/C46H64N5O8PS/c1-34(2)51(35(3)4)60(58-27-12-25-47)59-32-31-57-30-29-56-28-26-48-43(52)16-11-10-15-42-44-41(33-61-42)50(45(53)49-44)46(36-13-8-7-9-14-36,37-17-21-39(54-5)22-18-37)38-19-23-40(55-6)24-20-38/h7-9,13-14,17-24,34-35,41-42,44H,10-12,15-16,26-33H2,1-6H3,(H,48,52)(H,49,53)/t41-,42-,44-,60?/m0/s1
SMILES string
COc1ccc(cc1)C(N2C3CS[C@@H](CCCCC(=O)NCCOCCOCCOP(OCCC#N)N(C(C)C)C(C)C)C3NC2=O)(c4ccccc4)c5ccc(OC)cc5
InChI key
WSDQYLGSIUJJRU-ZMKGMYJVSA-N
product line
Proligo Reagents
assay
≥96% (31P-NMR)
suitability
conforms to structure for H-NMR
compatibility
configured for ABI
storage temp.
−20°C
Quality Level
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General description
Biotin phosphoramidite is a reagent used to directly incorporate a biotin label at the 5′ hydroxy group of the terminal nucleoside base through a p-aminophenethyl spacer group during oligonucleotide synthesis. Biotin-labelled oligonucleotides have become extremely popular in DNAcapture and detection applications, due to their very high-sensitivity and strong, specific binding affinity with avidin and streptavidin proteins. Biotinbased detection methods and are widely used by researchers. Biotin labelling is compatible with PCR and most hybridization techniques.
Key Features of Biotin Phosphoramidite
Key Features of Biotin Phosphoramidite
- Behaves like any standard phosphoramidite on the DNA synthesizer
- Readily soluble in acetonitrile
- Biotin compound has a dimethoxytrityl (DMT) group on the ring structure,which allows coupling-yield determination and trityl-selective purification
- A long, water-compatible linker arm ensures maximum sensitivity
- Provides a coupling efficiency of at least 95%The product is configured for ABI™ Synthesizers
Legal Information
ABI is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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