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product name
StableCell™ MEM, With Earle′s salts, stable glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
质量水平
无菌性
sterile-filtered
形式
liquid
技术
cell culture | mammalian: suitable
杂质
endotoxin, tested
组分
sodium pyruvate: no
HEPES: no
Hanks’ salts (2% CO2): no
NaHCO3: yes
Earle’s salts (5% CO2): no
stable glutamine: yes
phenol red: yes
L-glutamine: no
glucose: yes
运输
ambient
储存温度
2-8°C
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一般描述
由 Harry Eagle 开发的最低必需培养基(MEM)是所有合成细胞培养基中使用最广泛的培养基之一。
由 Harry Eagle 开发的最低必需培养基(MEM)是所有合成细胞培养基中使用最广泛的培养基之一。正常哺乳动物成纤维细胞和某些亚型的 HeLa 细胞的早期尝试培养表明,Eagle 基础培养基(BME)无法满足的他们的特殊营养需求。随后培养这些和其他细胞的研究表明,在 BME 中补充添加剂有利于更多种类的苛求细胞的生长。
MEM 包含了这些改进,包括更高浓度的氨基酸,因此培养基更接近于培养的哺乳动物细胞的蛋白质组成。MEM 已用于多种单层细胞的培养。在含有 Hanks 或 Earle 盐的配方中可选地补充非必需氨基酸,扩大了该培养基的用途。
MEM 包含了这些改进,包括更高浓度的氨基酸,因此培养基更接近于培养的哺乳动物细胞的蛋白质组成。MEM 已用于多种单层细胞的培养。在含有 Hanks 或 Earle 盐的配方中可选地补充非必需氨基酸,扩大了该培养基的用途。
应用
低限量 Eagle 培养基已用于小鼠成纤维细胞培养,用于体外细胞相容性测试。
其他说明
该产品不含 L-丙氨酸;L-天冬酰胺;L-谷氨酸;甘氨酸;L-脯氨酸;L-丝氨酸 和 L-谷氨酰胺。该产品补充了 L-Ala-L-Gln 二肽。这为细胞培养提供了更稳定的谷氨酰胺形式。游离氨基酸 L-谷氨酰胺在细胞培养中是不稳定的。
法律信息
StableCell is a trademark of Sigma-Aldrich Co. LLC
推荐
通常也和此产品一起购买
产品编号
说明
价格
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves
法规信息
动植物来源培养基
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Materials science & engineering. C, Materials for biological applications, 79, 550-562 (2017-06-21)
Recently, iron-based materials have been considered as candidates for the fabrication of biodegradable load-bearing implants. Alloying with palladium has been found to be a suitable approach to enhance the insufficient corrosion rate of iron-based alloys. In this work, we have
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Titanium biomaterials are widely used in the medical field due to their biocompatibility and excellent corrosion and mechanical resistance. However, these materials have no antibacterial properties. To obtain an antibacterial active surface, a nanostructure of Ti6Al4V alloy was created. This
Optimization of chemically defined cell culture media-replacing fetal bovine serum in mammalian in vitro methods
Toxicology in vitro, 24(4), 1053-1063 (2010)
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