Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
HEPES: no
Earle’s salts (5% CO2): yes
NaHCO3: no
phenol red: yes
L-glutamine: yes
sodium pyruvate: no
shipped in
ambient
storage temp.
2-8°C
General description
它含有19种氨基酸。必需氨基酸和非必需氨基酸；L-ala、L-asn、L-asp、L-glu、L-gly、L-pro和L-ser。可能跟适合于在血清浓度降低的情况下生长贴壁细胞，或作为开发无血清培养基的基础。
最小必需培养基Eagle包含糖、无机盐、水溶性维生素和必需氨基酸。它广泛用于许多细胞培养基研究的培养基配方。它是Eagle基础培养基（BME）的改良版本，大部分氨基酸的浓度是BME的两倍。
Application
最小必需培养基Eagle已用于培养成骨样细胞系MG-63、分散心室细胞和子宫内膜腺癌Ishikawa细胞。
Preparation Note
按照每升培养基9.6克粉末配制。
补充2.2 g/L碳酸氢钠。
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存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
商品
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
High-quality ultrapure water that is endotoxin-free is recommended for mammalian cell culture as it support cell viability and prevents contamination.
János Magyar et al.
European journal of pharmacology, 487(1-3), 29-36 (2004-03-23)
Concentration-dependent (10-1000 microM) effects of terpenoid phenol derivatives were studied on L-type Ca(2+) current in isolated canine and human ventricular cardiomyocytes using the whole-cell configuration of patch clamp technique. Carvacrol, thymol and eugenol suppressed peak Ca(2+) current at +5 mV
Atomic layer deposited TiO2 films on an equiatomic NiTi shape memory alloy for biomedical applications
Abbas A, et al.
Journal of alloys and compounds, 161282-161282 (2021)
Is it time to reinvent basic cell culture medium?
Turney J McKee et al.
American journal of physiology. Cell physiology, 312(5), C624-C626 (2017-02-24)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| M0643-50K | 04061834181491 |
| M0643-10L | 04061833993750 |
| M0643-1L | 04061837459504 |
| M0643-50L | 04061833993767 |
| M0643-10X1L | 04061835568802 |