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Merck
CN

M0894

Sigma-Aldrich

Eagle最低必需培养基

Alpha Modification, with L-glutamine and sodium pyruvate, without ribonucleosides, deoxyribonucleosides and sodium bicarbonate, powder, suitable for cell culture

别名:

MEM

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.71

质量水平

形式

powder

技术

cell culture | mammalian: suitable

组分

stable glutamine: no
Hanks’ salts (2% CO2): no
sodium pyruvate: yes
NaHCO3: no
phenol red: yes
glucose: yes
L-glutamine: yes
Earle’s salts (5% CO2): yes
HEPES: no

运输

ambient

储存温度

2-8°C

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一般描述

这是所提供的MEM配方中含量最丰富的变量。它含有所有21种正常氨基酸,其中一些的浓度有所增加。此外,它含有5种额外的维生素。

数量

按照每升培养基10.1克粉末配制。

重悬

补充2.2 g/L碳酸氢钠。

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Skin Sens. 1

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

分析证书(COA)

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Jason W Ashley et al.
The Journal of biological chemistry, 291(44), 23268-23281 (2016-10-30)
Macrophages are important in innate and adaptive immunity. Macrophage participation in inflammation or tissue repair is directed by various extracellular signals and mediated by multiple intracellular pathways. Activation of group VIA phospholipase A2 (iPLA2β) causes accumulation of arachidonic acid, lysophospholipids
Alexander J Nelson et al.
Journal of lipid research, 61(2), 143-158 (2019-12-11)
Phospholipases A2 (PLA2s) catalyze hydrolysis of the sn-2 substituent from glycerophospholipids to yield a free fatty acid (i.e., arachidonic acid), which can be metabolized to pro- or anti-inflammatory eicosanoids. Macrophages modulate inflammatory responses and are affected by Ca2+-independent phospholipase A2
Jun Zhang et al.
Therapeutic advances in chronic disease, 12, 2040622321995685-2040622321995685 (2021-08-31)
Previous studies reported that melatonin exerts its effect on mesenchymal stem cell (MSC) survival and differentiation into osteogenic and adipogenic lineage. In the current study we aimed to explore the effect of melatonin on osteoporosis and relevant mechanisms. Real-time qualitative
Kateřina Valentová et al.
International journal of molecular sciences, 19(8) (2018-08-12)
Silymarin, an extract from milk thistle (Silybum marianum) fruits, is consumed in various food supplements. The metabolism of silymarin flavonolignans in mammals is complex, the exact structure of their metabolites still remains partly unclear and standards are not commercially available.
Yuxuan Xiao et al.
Biochemical and biophysical research communications, 487(3), 640-645 (2017-04-25)
The meiotic G2/M1 transition is mostly regulated by posttranslational modifications, however, the cross-talk between different posttranslational modifications is not well-understood, especially in spermatocytes. Sumoylation has emerged as a critical regulatory event in several developmental processes, including reproduction. In mouse oocytes

商品

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

实验方案

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

粉末培养基及盐混合物极具吸湿性,应注意防潮。

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