M1302
M-MLV 逆转录酶
Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis
别名:
莫洛尼鼠白血病病毒逆转录酶
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关于此项目
Biological source:
Porcine intestinal mucosa
Recombinant:
expressed in E. coli
Concentration:
200 units/μL
biological source
Porcine intestinal mucosa
Quality Segment
recombinant
expressed in E. coli
form
liquid
usage
sufficient for 200 reactions
feature
dNTPs included: no, hotstart: no
concentration
200 units/μL
technique(s)
RT-PCR: suitable
color
colorless
input
purified RNA
shipped in
wet ice
storage temp.
−20°C
General description
莫洛尼鼠白血病病毒(M-MLV)逆转录酶从质粒表达M-MLV部分pol基因的大肠杆菌中分离获得。MoMLVRT由671个氨基酸组成。它是一种DNA聚合酶，可通过单链RNA、DNA或RNA-DNA杂合体（使用引物）合成互补的DNA链。
Application
M-MLV逆转录酶已用于：
- 制备cDNA文库或合成第一条cDNA链
- 2步法RT-PCR检测
- 实时定量聚合酶链反应(RT-qPCR)
- 逆转录
Features and Benefits
- 热稳定性逆转录酶在37℃时活性佳。
- 可用于制备长达7 kb的第一链cDNA。
Packaging
本品为含DTT 的10× M-MLV逆转录酶缓冲液。
Preparation Note
该酶从可在质粒上表达M-MLV pol基因的Escherichia coli 中纯化所得。
Other Notes
一单位本品可在10分钟内将1 nmol TTP整合进酸性可沉淀物质中。反应在37℃下进行，以poly(A)：oligo dT为模板：引物。
Legal Information
Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)