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M1302

M-MLV 逆转录酶

Name: M-MLV 逆转录酶
Brand: SIGMA

Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis

别名:

莫洛尼鼠白血病病毒逆转录酶

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关于此项目

化学文摘社编号:

9068-38-6

UNSPSC Code:

12352202

NACRES:

NA.55

MDL number:

MFCD00283754

Biological source:

Porcine intestinal mucosa

Recombinant:

expressed in E. coli

Concentration:

200 units/μL

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biological source

Porcine intestinal mucosa

Quality Level

200

recombinant

expressed in E. coli

form

liquid

usage

sufficient for 200 reactions

feature

dNTPs included: no, hotstart: no

concentration

200 units/μL

technique(s)

RT-PCR: suitable

color

colorless

input

purified RNA

shipped in

wet ice

storage temp.

−20°C

General description

莫洛尼鼠白血病病毒(M-MLV)逆转录酶从质粒表达M-MLV部分pol基因的大肠杆菌中分离获得。MoMLVRT由671个氨基酸组成。它是一种DNA聚合酶，可通过单链RNA、DNA或RNA-DNA杂合体（使用引物）合成互补的DNA链。

Application

M-MLV逆转录酶已用于：

制备cDNA文库或合成第一条cDNA链
2步法RT-PCR检测
实时定量聚合酶链反应(RT-qPCR)
逆转录

Features and Benefits

热稳定性逆转录酶在37℃时活性佳。
可用于制备长达7 kb的第一链cDNA。

Packaging

本品为含DTT 的10× M-MLV逆转录酶缓冲液。

Preparation Note

该酶从可在质粒上表达M-MLV pol基因的Escherichia coli 中纯化所得。

Other Notes

一单位本品可在10分钟内将1 nmol TTP整合进酸性可沉淀物质中。反应在37℃下进行，以poly(A)：oligo dT为模板：引物。

Legal Information

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

存储类别

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

常规特殊物品

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

0000472176

0000401710

0000279806

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