Merck
CN

M2378

Sigma-Aldrich

黏液蛋白 来源于猪胃

Type II

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别名:
MUC
CAS号:
EC 号:
MDL编号:
NACRES:
NA.61

生物来源

Porcine stomach

类型

Type II

形式

powder

组成

bound sialic acid, ≤1.2%

technique(s)

cell culture | mammalian: suitable

溶解性

1 M NaOH: soluble 20 mg/mL

UniProt登记号

储存温度

2-8°C

Gene Information

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一般描述

粘蛋白主要由碳水化合物单元组成,对结构结构和构象至关重要。粘蛋白的单体为640 kDa,蛋白质仅占分子量的20%。其中的碳水化合物成分附着在苏氨酸和丝氨酸侧链上。粘蛋白分为分泌型和跨膜粘蛋白。
黏液蛋白或黏液糖蛋白是哺乳动物黏液的主要大分子成分。它还被应用于评价细胞离心浓缩痰涂片检测抗酸杆菌的研究。

应用

来自猪胃的粘蛋白以用于:
  • 绘制定量小鼠粘膜炎症过程产生的粘蛋白2的标准曲线
  • 用作人体外消化模型中的消化液溶液组分
  • 用作模拟唾液的组分

猪胃黏液素已应用于 铜绿假单胞菌半乳糖特异性凝集素PA-IL结合位点的研究。

生化/生理作用

粘蛋白在药物递送应用中具有广泛的药理学应用。粘蛋白在炎症过程中起着保护作用。粘蛋白合成失调有可能引起肿瘤发生和慢性炎症。
黏液形成所有上皮表面的保护层。黏液的主要结构成分是形成凝胶的黏液素。覆盖肠上皮的黏液层以MUC2粘蛋白为中心分子。MUC2是由杯状细胞产生。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What does the "Type" designation in Mucin from porcine stomach mean?

    The type designation does not have any particular biological significance.  It is used to emphasize differences in the listings of this product.

  6. What are the differences between Mucin from porcine stomach Products M2378 and M1778?

    Product No. M2378 is a crude mucin preparation. Product No. M1778 is a partially purified powder prepared according to the method described in Glenister, D.A. and Salmon. K. Microbial Ecol. in Health & Disease 1, 31, (1988). The amount of bound sialic acids are also different. Please refer to the specification sheets on our website.

  7. Is Product M2378, Mucin from porcine stomach, a purified preparation of mucin?

    No. This product is a crude preparation of mucin; it is a mixture of glycoproteins.  It is obtained by digestion of hog stomach with pepsin, followed by precipitation and other steps.

  8. How can I solubilize Product M2378, Mucin from porcine stomach?

    This crude mucin may not be readily soluble in solution. At 20 mg/mL in 1 M sodium hydroxide, turbid suspensions are observed. To try to increase the solubility, one might try further dilution and sonication, but we have not tried this in our labs.

  9. What is the molecular weight of Product M2378, Mucin from porcine stomach?

    This product is a crude mixture of glycoproteins. The sugar and amino acid composition of the mucin has not been determined.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Food Research International, 114, 30-37 (2018)
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Nature Reviews. Cancer, 9(12), 874-874 (2009)
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ACS infectious diseases, 7(5), 1126-1142 (2020-11-13)
It is widely accepted that the pathogen Clostridioides difficile exploits an intestinal environment with an altered microbiota, but the details of these microbe-microbe interactions are unclear. Adherence and colonization of mucus has been demonstrated for several enteric pathogens and it

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Review article and products for sialic acid synthesis and signaling.

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