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安全信息

M2520

Sigma-Aldrich

培养基 199

HEPES Modification, with Earle′s salts, L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

别名:

M199 Medium, TCM 199

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.75

质量水平

500

形式

powder

技术

cell culture | mammalian: suitable

组分

L-glutamine: yes
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
sodium pyruvate: no
HEPES: 25 mM

运输

ambient

储存温度

2-8°C

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相关类别

一般描述

培养基199是维生素、氨基酸和其他因素的组合，最初开发为用于原代外植体培养的完全确定的培养基配方。这种培养基添加适当的补充剂时具有广泛的适用性，特别是对于非转化细胞。培养基 199 广泛用于疫苗生产、原代胰腺外植体和晶状体组织的体外培养。

应用

培养基199已用于：

  • 在成熟前保持卵丘-卵母细胞复合体（COC），并培养这些卵母细胞在体外成熟
  • 在德国荷斯坦奶牛卵子采集过程中清洗抽吸管
  • 培养大鼠心脏内皮细胞

数量

配制成每升培养基含 14.7 克粉末。

重悬

补充 2.2 g/L 碳酸氢钠。

补充剂

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价格

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产品编号
说明
价格

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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Mao Ye et al.
Scientific reports, 4, 4681-4681 (2014-04-16)
The highly specialized endothelial cells in brain capillaries are a key component of the blood-brain barrier, forming a network of tight junctions that almost completely block paracellular transport. In contrast to vascular endothelial cells in other organs, we show that
Jarosław Wieczorek et al.
Animals : an open access journal from MDPI, 10(4) (2020-04-16)
The laparoscopic method of recovering oocytes in goats and sheep is one of the minimally invasive methods used in the biotechnology of animal reproduction. It allows for good quality oocytes that are suitable for in vitro maturation and fertilization to
In vitro nuclear maturation of bitch oocytes in the presence of polyvinyl-pyrrolidone
Animal Reproduction, 3(1), 70-75 (2018)
Sait Sendag et al.
Animal reproduction science, 106(1-2), 208-214 (2008-02-26)
The goal of the present study was to compare the ovarian response, oocyte yields per animal, and the morphological quality of oocytes collected by ultrasound guided follicular aspiration from Holstein cows treated either with FSH or eCG. Twenty four normal
L González-Fernández et al.
Reproduction, fertility, and development, 30(2), 297-306 (2017-07-07)
Equine cumulus-oocyte complexes (COCs) are classified as compact (cCOC) or expanded (eCOC) and vary in their meiotic competence. This difference could be related to divergent glucose metabolism. To test this hypothesis in the present study, eCOCs, cCOCs and expanded or
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商品

Medium 199 - M199 Formulations

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Calcium in Cell Culture

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

钙在细胞培养中的应用

钙是一种离子稳定的二价阳离子，在细胞培养基中具有有益和有毒的双重属性，可作为一系列细胞培养基的组成部分。

实验方案

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

粉末培养基制备说明

粉末培养基及盐混合物极具吸湿性，应注意防潮。

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