M2634
苹果酸脱氢酶 来源于猪心脏
buffered aqueous glycerol solution, 600-1000 units/mg protein (biuret)
别名:
L-苹果酸: NAD+ 氧化还原酶, MDH, Malate Dehydrogenase
产品名称
苹果酸脱氢酶 来源于猪心脏, buffered aqueous glycerol solution, 600-1000 units/mg protein (biuret)
form
buffered aqueous glycerol solution
specific activity
600-1000 units/mg protein (biuret)
cation traces
NH4+: ≤10 μg/mg protein
foreign activity
Glutamic-Oxalacetic Transaminase ≤0.01%
Glutamic-Pyruvic Transaminase ≤0.01%
shipped in
wet ice
storage temp.
2-8°C
Quality Level
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Biochem/physiol Actions
真核细胞含有两种不同的苹果酸脱氢酶同工酶：线粒体同工酶（m-MDH）和可溶性或细胞质同工酶（s-MDH）。该产品由线粒体同工酶形式组成。
该酶可催化以下反应：草酰乙酸+β-NADH→ L-苹果酸+β-NAD
该酶可催化以下反应：草酰乙酸+β-NADH→ L-苹果酸+β-NAD
通过在25℃下用5′-磷酸吡哆醛处理可以可逆地对苹果酸脱氢酶进行灭活。
General description
线粒体同工酶
苹果酸脱氢酶是线粒体同工酶，也是三羧酸循环中的重要催化剂。
Other Notes
在pH7.5,25℃条件下，一个单位每分钟可将1.0 μmole的草酰乙酸和β-NADH转化为 L苹果酸和β-NAD。
Physical form
溶于含50％甘油的0.05M磷酸钾缓冲液（pH7.5）中
Application
采用苹果酸脱氢酶对银杏提取物对寄主钉螺的软体动物的杀灭作用进行了比较研究。 它还被用于研究早期饲养限制对肉鸡代谢程序和补偿性生长的影响。
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
低风险生物材料
此项目有
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S S Chen et al.
The Biochemical journal, 151(2), 297-303 (1975-11-01)
1. Pig heart mitochondrial malate dehydrogenase incubated with pyridoxal 5'-phosphate at pH 8.0 and 25 degrees C gradually loses activity. Such inactivation can be largely reversed by dialysis or by addition of L-lysine or L-cysteine, and can be made permanent
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Pesticide Biochemistry and Physiology, 89, 237-241 (2007)
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Journal of pharmacological sciences, 112(3), 320-326 (2010-02-23)
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实验方案
Spectrophotometric assay evaluates malic dehydrogenase activity using bovine heart enzyme with critical histidine residue at active site.
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