Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
L-glutamine: no
phenol red: no
HEPES: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: no
shipped in
ambient
storage temp.
2-8°C
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General description
培养基199是维生素、氨基酸和其他因素的组合，最初开发为用于原代外植体培养的完全确定的培养基配方。这种培养基添加适当的补充剂时具有广泛的适用性，特别是对于非转化细胞。培养基 199 广泛用于疫苗生产、原代胰腺外植体和晶状体组织的体外培养。
Application
培养基199已用于：
- 培养原代人脐静脉内皮细胞（HUVEC），以用于细胞活力检测
- 冲洗小母猪子宫角，从子宫恢复第12天妊娠
- 培养腹膜细胞和永生化人间皮细胞（MeT-5A和ATCC-CRL-9444）
Preparation Note
补充0.1克/升左旋谷氨酰胺、2.2克/升碳酸氢钠。
配制成每升培养基中含 9.4 克粉末。
存储类别
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Successful pregnancy establishment in mammals depends on proper embryo-maternal communication. Prokineticin 1 (PROK1) is a secretory protein that exerts pleiotropic functions in various tissues. Despite the studies that have primarily been performed with human cell lines and mice, the function
商品
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| M3769-50L | 04061834050568 |
| M3769-10X1L | 04061834050551 |
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