M4287
丝裂霉素 C 来源于头状链霉菌
powder, suitable for cell culture, BioReagent
别名:
Mitomycin
选择尺寸
关于此项目
产品名称
丝裂霉素 C 来源于头状链霉菌, powder, BioReagent, suitable for cell culture
InChI
1S/C15H18N4O5/c1-5-9(16)12(21)8-6(4-24-14(17)22)15(23-2)13-7(18-13)3-19(15)10(8)11(5)20/h6-7,13,18H,3-4,16H2,1-2H3,(H2,17,22)/t6-,7+,13+,15-/m1/s1
SMILES string
[H][C@]12CN3C4=C([C@@H](COC(N)=O)[C@@]3(OC)[C@@]1([H])N2)C(=O)C(N)=C(C)C4=O
InChI key
NWIBSHFKIJFRCO-WUDYKRTCSA-N
biological source
Streptomyces caespitosus
product line
BioReagent
form
powder
technique(s)
cell culture | mammalian: suitable
color
gray
purple/blue
(1) 3.2, (2) 6.5
solubility
H2O: 4 mL/vial, clear to slightly hazy, blue to purple (Stock solutions should be filter sterilized and stored at 2-8 °C in the dark.)
antibiotic activity spectrum
Gram-negative bacteria
Gram-positive bacteria
mode of action
DNA synthesis | interferes
storage temp.
2-8°C
Quality Level
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Application
Biochem/physiol Actions
抗菌谱：丝裂霉素C具有很强的抗肿瘤活性，尤其是对艾氏腹水瘤细胞，对革兰氏阳性和革兰氏阴性细菌具有很强的杀菌作用。
Disclaimer
General description
化学结构：氮丙啶
Preparation Note
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Carc. 2
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is the difference between Mitomycin C products M4287 and M0503?
M4287 has been cell culture tested; M0503 has not been cell culture tested.
How should solutions of Mitomycin C, Product M4287, be stored?
Solutions of this product should not be stored frozen. Solutions can be stored at 2-8°C, and should be used within 1-2 weeks. Solutions are light sensitive, but the powder is not.
How should the product be dissolved?
Mitomycin C is soluble at 0.5 mg/mL in water, yielding a clear blue solution.
At what concentration can Mitomycin C, Product M4287, be used with embryonic stem (ES) cells?
Treatment of the feeder layer with media containing 100 micrograms/mL mitomycin at 37°C for 30 minutes blocked replication of ES cells.
What is the recommended concentration of Mitomycin C, Product M4287, should I use for eukaryote cell culture?
A concentration between 10-50 micrograms/mL is typically used.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
商品
Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic
抗生素杀灭曲线是一项剂量响应实验，通过逐渐增大施加在哺乳动物细胞中的备选抗生素剂量进行测定。
实验方案
This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.
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Instructions
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