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Merck
CN

M4526

Eagle最低必需培养基

Alpha Modification, with sodium bicarbonate and Earl's salts, without ʟ-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture

别名:

αMEM, MEM

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UNSPSC Code:
12352207
NACRES:
NA.71

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产品名称

Eagle最低必需培养基, Alpha Modification, with sodium bicarbonate, without L-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture

form

liquid

sterility

sterile-filtered

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

Earle’s salts (5% CO2): yes
sodium pyruvate: yes
glucose: yes
L-glutamine: no
HEPES: no
Hanks’ salts (2% CO2): no
phenol red: yes
NaHCO3: yes
stable glutamine: no

shipped in

ambient

storage temp.

2-8°C

Quality Level

400

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相关类别

Application

最小必需培养基Eagle已用于维持：
  • 小鼠未成熟的卵母细胞络合物
  • 人类间充质干细胞（MSC）
  • 小鼠成骨前细胞系

General description

最小必需培养基Eagle（MEM）是一种合成细胞培养基，由Harry Eagle开发。它有更高浓度的氨基酸，所以培养基更接近培养哺乳动物细胞的蛋白质组成。在含有Hanks或Earle盐的配方中选择补充非必需氨基酸，可增强该培养基的用途。Earle平衡盐的MEM修饰也称为αMEM，含有非必需氨基酸、丙酮酸钠和其他维生素。
这是所提供的MEM配方中最丰富的变体。它含有所有21种正常氨基酸，其中一些的浓度有所增加。此外，它还含有5种额外的维生素。

Preparation Note

添加 0.292g/L L-谷氨酰胺。

存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

动植物来源培养基
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
RNBM0562
RNBL7706
RNBL2659
RNBL5431
RNBL0707

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Journal of molecular histology, 51(3), 241-250 (2020-05-14)
Using a large-scale quantitative mesenchymal stem cells (MSCs) membrane proteomics analysis, we identified a large group of ciliary proteins in the MSCs membrane fraction, which prompted us to examine the cilia, intricate organelles that were originally discovered approximately 100 years ago.
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Yeast display immunoprecipitation is a combinatorial library screening platform for the discovery and engineering of antibodies against membrane proteins using detergent-solubilized membrane fractions or cell lysates as antigen sources. Here, we present the extension of this method for the screening
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