产品名称
Eagle最低必需培养基, With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
form
powder
technique(s)
cell culture | mammalian: suitable
components
glucose: yes
Hanks’ salts (2% CO2): yes
L-glutamine: yes
Earle’s salts (5% CO2): no
NaHCO3: no
phenol red: yes
sodium pyruvate: no
stable glutamine: no
HEPES: no
shipped in
ambient
storage temp.
2-8°C
General description
Eagle最低必需培养基(MEM)是Harry Eagle开发的人工合成细胞培养基。培养基含有较高浓度的氨基酸，因此比较接近哺乳动物培养细胞的蛋白质浓度。在含有Hanks′或Earle′s盐的配方中选择补充非必需氨基酸，可扩大该培养基的用途。去除钙成分后，可用于悬浮细胞培养。它是经典MEM配方的改良版。厄尔′盐类已被汉克′盐类所取代。由于碳酸氢盐浓度显著降低，因此该介质具有显著降低的缓冲能力。
Application
Eagle最低必需培养基已用于：
- 作为Hank′s MEM(HMEM)-PIPES缓冲液成分，用于小鼠中过敏毒素(anaphylatoxin)诱导耳肿的引发
- 作为小鼠海马体切片培养基(SCM)组分
- 培养豚鼠Müller细胞
Preparation Note
按照每升培养基10.6克粉末配制。
补充0.35 g/L碳酸氢钠。
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存储类别
11 - Combustible Solids
wgk
WGK 1
实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
商品
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
The activation of IL-8 receptors in cultured guinea pig Muller glial cells is modified by signals from retinal pigment epithelium
Iwona M G, et al.
Journal of Neuroimmunology, 161, 49-60 (2005)
Mast cell anaphylatoxin receptor expression can enhance IgE-dependent skin inflammation in mice
Beatrix S, et al.
The Journal of Allergy and Clinical Immunology, 131(2), 541-548 (2013)
Anthony Dudilot et al.
Current biology : CB, 30(7), 1167-1176 (2020-02-23)
Synaptic plasticity, with its two most studied forms, long-term potentiation (LTP) and long-term depression (LTD), is the cellular mechanism underlying learning and memory. Although it has been known for two decades that bidirectional synaptic plasticity necessitates a corresponding bidirectional regulation
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| M4642-50K | 04061834171935 |
| M4642-10L | 04061834059097 |
| M4642-50L | 04061834059103 |