M4812
Anti-Myosin Va (LF-18) antibody produced in rabbit
~0.4 mg/mL, affinity isolated antibody, buffered aqueous solution
别名:
Anti-GS1, Anti-MYH12, Anti-MYO5, Anti-MYR12
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关于此项目
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ARR, IHC (p), WB
Citations:
14
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 190 kDa
species reactivity
chicken, rat
enhanced validation
independent
Learn more about Antibody Enhanced Validation
concentration
~0.4 mg/mL
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using rat and chicken cerebellum sections, microarray: suitable, western blot: 1:1,000 using a rat brain extract
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MYO5A(4644)
mouse ... Myo5a(17918)
rat ... Myo5a(25017)
General description
Anti-myosin Va (LF-18) recognizes myosin Va (190kDa). Myosin 5a is encoded by the gene mapped to human chromosome 15q21.2. The encoded protein is a member of the unconventional myosin family. It is a two-headed motor containing two identical heavy chains and multiple light chains. Myosin Va is widely expressed, but at high levels in the brain, testes and melanocytes.
Immunogen
synthetic peptide located near the C-terminus of chicken myosin Va (amino acids 1782-1799 with N-terminally added lysine) conjugated to KLH. This sequence is identical in human, mouse and rat.
Application
Anti-Myosin Va (LF-18) antibody produced in rabbit has been used in:
- Western blot.
- In situ proximity ligation assay (PLA).
- Glutathione S-transferase (GST)-spinophilin co-sedimentation assay.
Biochem/physiol Actions
Myosin 5a is an actin-based molecular motor responsible for transport of secretory granules in various cell systems, including insulin granule. Additionally, it also mediates the transport of cargo such as cell-surface receptors, pigment and organelles like endoplasmic reticulum to the plasma membrane. The encoded protein facilitates pigmentation and synaptic activity in the central nervous system. Genetic variations in the gene are associated with the development of immunological and neurological diseases, including Griscelli syndrome type 1 and Elejalde syndrome.
Physical form
Solution in 0.01 M phosphate buffered saline containing 1% BSA and 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8°C for up to one month. For prolonged storage, freeze in working aliquots at -20°C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Other Notes
In order to obtain best results in different techniques and preparations we recommend determining optimal working dilutions by titration test.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
常规特殊物品
此项目有
The molecular motor Myosin Va interacts with the cilia-centrosomal protein RPGRIP1L
Assis LH, et al.
Scientific Reports (2017)
Genome-Wide Meta-Analysis of Sciatica in Finnish Population
Lemmela S, et al.
PLoS ONE, 11 (2016)
Ianina L Conte et al.
Journal of cell science, 129(3), 592-603 (2015-12-18)
Weibel-Palade body (WPB)-actin interactions are essential for the trafficking and secretion of von Willebrand factor; however, the molecular basis for this interaction remains poorly defined. Myosin Va (MyoVa or MYO5A) is recruited to WPBs by a Rab27A-MyRIP complex and is
Identification and validation of novel spinophilin-associated proteins in rodent striatum using an enhanced ex vivo shotgun proteomics approach.
Baucum AJ 2nd, et al.
Molecular and Cellular Proteomics, 9, 1243-1259 (2010)
Roles for myosin Va in RNA transport and turnover.
McCaffrey MW and Lindsay AJ.
Biochemical Society Transactions, 40, 1416-1420 (2012)
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