M5062
Anti-Myosin Va (LE-16) antibody produced in rabbit
~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution
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关于此项目
Conjugate:
unconjugated
Clone:
polyclonal
Application:
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
microarray
western blot
microarray
western blot
Species reactivity:
rat, chicken
Citations:
4
Technique(s):
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using rat and chicken cerebellum sections
microarray: suitable
western blot: 1:500 using a rat brain extract
microarray: suitable
western blot: 1:500 using a rat brain extract
Uniprot accession no.:
产品名称
Anti-Myosin Va (LE-16) antibody produced in rabbit, ~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 190 kDa
species reactivity
rat, chicken
enhanced validation
independent
Learn more about Antibody Enhanced Validation
concentration
~0.5 mg/mL
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using rat and chicken cerebellum sections
microarray: suitable
western blot: 1:500 using a rat brain extract
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MYO5A(4644)
mouse ... Myo5a(17918)
rat ... Myo5a(25017)
Application
Anti-Myosin Va (LE-16) antibody produced in rabbit is used in immunoblotting and immunohistochemistry.
Biochem/physiol Actions
Myosin Va is implicated in the regulation of vesicle trafficking in neurons and melanocytes. It regulates melanosome distribution along microfilaments. It is found in association with the centrosome at all stages of the cell cycle. In the interphase stage, myosin Va is found in pericentriolar material. During cell division, it is found in the cytoplasm and concentrates in a trail between migrating centrioles and in the mitotic spindle poles and spindle fibers.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Myosin Va (p190) is a member of the unconventional class of myosins, distinct from both the myosins I and myosin II. It is present in neuronal and non neuronal cells of the brain. Class V myosins are widely expressed actin-based motors. Class V myosins have two motor head domains typical of myosins, and an extended regulatory neck domain with six tandem IQ domains that bind multiple calmodulin light chains. In addition, myosin V contains a unique 400 amino acids globular tail domain that may direct myosin V to its target or determine the cargo to which it binds.
Immunogen
synthetic peptide located near the C-terminus of chicken myosin Va (amino acids 1705-1720 with N-terminally added lysine) conjugated to KLH. This sequence is identical in human, mouse and rat.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Regulated conformation of myosin V
Wang F, et al.
The Journal of Biological Chemistry, 279(4), 2333-2336 (2004)
High affinity binding of brain myosin-Va to F-actin induced by calcium in the presence of ATP
Tauhata SBF, et al.
The Journal of Biological Chemistry, 276(43), 39812-39818 (2001)
A millennial myosin census
Berg JS, et al.
Molecular Biology of the Cell, 12(4), 780-794 (2001)
Katharina N Richter et al.
Scientific reports, 8(1), 14838-14838 (2018-10-06)
Protein copy numbers can be measured by biochemical methods ranging from quantitative Western Blotting to several mass spectrometry approaches. Such methods only provide average copy numbers, obtained over large cell numbers. However, copy number estimates for single cells or single
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