M5257
Malic Dehydrogenase (oxalacetate-decarboxylating) from chicken liver
ammonium sulfate suspension, 10-30 units/mg protein (modified Warburg-Christian)
别名:
L-Malate:NADP+ oxidoreductase-(oxalacetate-decarboxylating), Malate Dehydrogenase (oxalacetate-decarboxylating), Malic Enzyme
生物来源
chicken liver
表单
ammonium sulfate suspension
比活
10-30 units/mg protein (modified Warburg-Christian)
储存温度
2-8°C
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一般描述
Malic dehydrogenase (MDH) exists as two isoforms within eukaryotic cells, one that is expressed in the mitochondria and functions in the TCA cycle and one in the cytoplasm that converts malate from the mitochondria back into oxaloacetate.
应用
Malic dehydrogenase has been used in a study to assess the dietary manganese requirement of juvenile yellow catfish (Pelteobagrus fulvidraco) and effects on whole body mineral composition and hepatic intermediary metabolism. It has also been used in a study to investigate the establishment and biological characterization of a fibroblast cell line from the Langshan chicken.
生化/生理作用
Expression of malic dehydrogenase in chicken liver is induced upon feeding and decreases in response to starvation.
外形
Suspension in 2.9 M (NH4)2SO4 solution containing 10 mM potassium phosphate, 0.5 mM 2-mercaptoethanol, 10 mM manganese chloride, and 3 mM Na4EDTA, pH 6.0
其他说明
One unit will convert 1.0 μmole of L-malate and NADP to pyruvate, CO2, and NADPH per min at pH 7.4 at 25 °C.
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
低风险生物材料
此项目有
Dietary manganese requirement of juvenile yellow catfish Pelteobagrus fulvidraco, and effects on whole body mineral composition and hepatic intermediary metabolism
Tan, X., et al.
Aquaculture (Amsterdam, Netherlands), 326, 68-73 (2012)
K N Rao et al.
Gastroenterology, 113(1), 238-248 (1997-07-01)
We showed previously that the peroxisome proliferators di(2-ethylhexyl)phthalate (DEHP), clofibrate, and 4-chloro-6-(2,3 xylidino)-2-pyrimidinylthio (N-beta-hydroxyl)acetamide (BR931) alter hepatic sex steroid metabolism and receptor expression during induction of hepatic hyperplasia and hepatocellular carcinoma (HCC) in rats. The aim of this study was
Synthesis and Degradation of Malic Enzyme in Chick Liver.
Silpananta, P
The Journal of Biological Chemistry, 264, 5754-5761 (1971)
Establishment and biological characterization of fibroblast cell line from the Langshan chicken
Guan, W., et al.
Cell Proliferation, 42, 157-163 (2010)
Mariel C Gerrard Wheeler et al.
The FEBS journal, 276(19), 5665-5677 (2009-09-04)
The Arabidopsis thaliana genome contains four genes encoding NADP-malic enzymes (NADP-ME1-4). Two isoenzymes, NADP-ME2 and NADP-ME3, which are shown to be located in the cytosol, share a remarkably high degree of identity (90%). However, they display different expression patterns and
商品
Instructions for working with enzymes supplied as ammonium sulfate suspensions
以硫酸铵悬浮液形式提供的酶的使用指南
相关内容
QC Methods
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