M5257
Malic Dehydrogenase (oxalacetate-decarboxylating) from chicken liver
ammonium sulfate suspension, 10-30 units/mg protein (modified Warburg-Christian)
别名:
L-Malate:NADP+ oxidoreductase-(oxalacetate-decarboxylating), Malate Dehydrogenase (oxalacetate-decarboxylating), Malic Enzyme
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-834-8
MDL number:
Specific activity:
10-30 units/mg protein (modified Warburg-Christian)
Biological source:
chicken liver
biological source
chicken liver
form
ammonium sulfate suspension
specific activity
10-30 units/mg protein (modified Warburg-Christian)
storage temp.
2-8°C
General description
Malic dehydrogenase (MDH) exists as two isoforms within eukaryotic cells, one that is expressed in the mitochondria and functions in the TCA cycle and one in the cytoplasm that converts malate from the mitochondria back into oxaloacetate.
Application
Malic dehydrogenase has been used in a study to assess the dietary manganese requirement of juvenile yellow catfish (Pelteobagrus fulvidraco) and effects on whole body mineral composition and hepatic intermediary metabolism. It has also been used in a study to investigate the establishment and biological characterization of a fibroblast cell line from the Langshan chicken.
Biochem/physiol Actions
Expression of malic dehydrogenase in chicken liver is induced upon feeding and decreases in response to starvation.
Physical form
Suspension in 2.9 M (NH4)2SO4 solution containing 10 mM potassium phosphate, 0.5 mM 2-mercaptoethanol, 10 mM manganese chloride, and 3 mM Na4EDTA, pH 6.0
Other Notes
One unit will convert 1.0 μmole of L-malate and NADP to pyruvate, CO2, and NADPH per min at pH 7.4 at 25 °C.
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
此项目有
Synthesis and Degradation of Malic Enzyme in Chick Liver.
Silpananta, P
The Journal of Biological Chemistry, 264, 5754-5761 (1971)
A R Ashton
Archives of biochemistry and biophysics, 345(2), 251-258 (1997-10-06)
NADP-malic enzyme (NADP-ME, EC 1.1.1.40) was purified to near-homogeneity from leaves of the C4 dicot Flaveria bidentis and shown to possess intrinsic NAD-dependent malic enzyme activity. The NAD-dependent activity is optimal at pH 7.5 and in the presence of Mn2+.
Dietary manganese requirement of juvenile yellow catfish Pelteobagrus fulvidraco, and effects on whole body mineral composition and hepatic intermediary metabolism
Tan, X., et al.
Aquaculture (Amsterdam, Netherlands), 326, 68-73 (2012)
Establishment and biological characterization of fibroblast cell line from the Langshan chicken
Guan, W., et al.
Cell Proliferation, 42, 157-163 (2010)
Mariel C Gerrard Wheeler et al.
The FEBS journal, 276(19), 5665-5677 (2009-09-04)
The Arabidopsis thaliana genome contains four genes encoding NADP-malic enzymes (NADP-ME1-4). Two isoenzymes, NADP-ME2 and NADP-ME3, which are shown to be located in the cytosol, share a remarkably high degree of identity (90%). However, they display different expression patterns and
商品
Instructions for working with enzymes supplied as ammonium sulfate suspensions
以硫酸铵悬浮液形式提供的酶的使用指南
相关内容
QC Methods
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