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Merck
CN

M5755

MOPS-EDTA-乙酸钠 (MESA)

powder for RNA electrophoresis buffers

别名:

MESA缓冲液

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关于此项目

NACRES:
NA.25
UNSPSC Code:
12161700

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产品名称

MOPS-EDTA-乙酸钠 (MESA), powder for RNA electrophoresis buffers

grade

Molecular Biology

product line

BioReagent

form

powder

technique(s)

electrophoresis: suitable

pH

6.8-7.2(reconstituted per directions)

solubility

H2O: soluble, faintly yellow

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

room temp

Quality Level

200

相关类别

General description

MOPS-EDTA-乙酸钠（MESA）是Northern印迹程序之前RNA电泳最常用的运行缓冲液。 对于最大值分离度，建议施加5 V/cm的电压。

Preparation Note

1x MESA缓冲液含有：
  • 40 mM MOPS
  • 10 mM 乙酸钠
  • 1 mM EDTA (pH 8.3)
将瓶中的全部内容物溶解在最终体积为1L的分子生物学级水中。 这产生了10X MESA贮备液，可以根据需要进一步稀释。

Application

适合在制备甲醛-琼脂糖凝胶和RNA电泳相关的运行缓冲液时使用。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000439909
0000439909
0000383887
0000257463
0000344083

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Kathryn M Jones et al.
Journal of bacteriology, 184(9), 2491-2499 (2002-04-12)
Two operons have been cloned from Anabaena sp. strain PCC 7120 DNA, each of which encodes the three core subunits of distinct mitochondrial-type cytochrome c oxidases. The two operons are only 72 to 85% similar to one another at the
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 7-7 (1989)
Molecular techniques for detection of gene expression in neuroimmunology.
Cowan, E.P., and Dhib-Jalbut, S.S.
Methods in Neuroscience, 24, 41-41 (1995)
Xueyu Xiang et al.
Developmental biology, 275(2), 343-355 (2004-10-27)
Recently, we cloned and sequenced the cDNA of allurin, a sperm chemoattractant isolated from the jelly of Xenopus laevis eggs [Proc. Natl. Acad. Sci. U.S.A. 78 (2001) 11205]. In this report, we demonstrate that allurin mRNA is expressed almost exclusively
Electrophoresis in agarose and acrylamide gels.
R C Ogden et al.
Methods in enzymology, 152, 61-87 (1987-01-01)
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实验方案

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

TAE and TBE Running Buffers Recipe & Video

TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.

Northern和Southern印迹实验方案及简介

Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

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